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高效液相色谱法检测定温州早茶中儿茶素含量

包箐箐 向铮 林崇良

中国药业2011,Vol.20Issue(22):50-51,2.
中国药业2011,Vol.20Issue(22):50-51,2.

高效液相色谱法检测定温州早茶中儿茶素含量

Determination of Tea Catechin from Wenzhou by HPLC

包箐箐 1向铮 2林崇良1

作者信息

  • 1. 温州医学院附属第一医院,浙江,温州,325000
  • 2. 温州医学院药学院,浙江,温州,325035
  • 折叠

摘要

Abstract

Objective To establish a method for the simultaneous determination of epigallocatechin galiate (EGCG) and epigallocatechin (EGG) in tea and to quantify two ingredients in four tea breeds from Wenzhou. Methods The study was achieved using a Zorbax Eclipse XDB-Cii column (150 mm x4. 6 mm, 5 (im) with gradient elution system of methanol - water containing 0.1% formic acid. The flow rale was 0. 8 mL/min, the column temperature was 25 V and the elution were detected at 270 nm. Results Under the selected analytical condition, the content of the analytes was good linear with their peak areas. The content of EGCC and EGC was at higher level. Conclusion The method is applicable for the determination of catechins from Wenzhou tea.

关键词

温州早茶/儿茶素/高效液相色谱法

Key words

Wenzhou tealcatechinlHPLC

分类

医药卫生

引用本文复制引用

包箐箐,向铮,林崇良..高效液相色谱法检测定温州早茶中儿茶素含量[J].中国药业,2011,20(22):50-51,2.

中国药业

OACSTPCD

1006-4931

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