中国组织工程研究与临床康复2011，Vol.15Issue(47)：8821-8824,4.DOI:10.3969/j.issn.1673-8225.2011.47.020
丝素蛋白/壳聚糖三维支架材料的制备方法
Preparation of three-dimensional silk fibroin/chitosan scaffolds
摘要
Abstract
BACKGROUND: Many studies have shown that silk fibroin (SF) and chitosan (CS) as natural polymer materials are non-toxic and tasteless, and they have good physical and chemical properties and biological characteristics.OBJECTIVE: To discuss the preparation methods of three-dimensional SF/CS scaffolds which meet the requirements of cartilage tissue engineering scaffolds.METHODS: SF and CS were mixed at different ratios such as 3 to 1, 1 to 1, 1 to 3 and 0 to 3, then to make composite materials.In order to find out the best mixing ratio of SF and CS, the following indexes including pore size, porosity, the water absorption and loss rate in hot water were determined.RESULTS AND CONCLUSION: It was more suitable to meet the requirements when the mixed ratio of SF to CS was 1 to 1. The pore size was 90-280 μm and the average pore size was 151.72 μm. The porosity was (92.72±4.78)%. The water absorption was (92.72±4.78)%. The loss rate in hot water after crosslinking was decreased compared before crosslinking, and there was a significant difference (P < 0.05). The mixed rati o of SF to CS is at 1 to 1 meeting the physicochemical requirements of cartilage tissue engineering scaffolds. The SF/CS composite material is expected to be an ideal scaffold material for cartilage tissue engineering.关键词
丝素蛋白/壳聚糖/支架/组织工程/材料分类
医药卫生引用本文复制引用
佘荣峰,邓江,黄文良,苑成,董志军..丝素蛋白/壳聚糖三维支架材料的制备方法[J].中国组织工程研究与临床康复,2011,15(47):8821-8824,4.基金项目
贵州省科学技术基金项目(黔科合J 字[2009]2172 号),课题名称:SF-CS 复合诱导骨髓间充质干细胞修复兔膝关节软骨缺损的研究 （黔科合J 字[2009]2172 号）
贵州省社会攻关项目(黔科合SY 字[2010]3101 号),课题名称:SF-CS-nHA 支架与基因修饰后BMSCs 构建人工骨种植体的成骨作用研究. （黔科合SY 字[2010]3101 号）
