广东医学2011，Vol.32Issue(24)：3157-3160,4.
S-腺苷甲硫氨酸对结直肠癌细胞生物学行为的影响
Effects of methylation agent S-adenosyl-L-methionine on the biological behaviors of colorectal cancer cells
摘要
Abstract
Objective To evaluate the effects of methylation agent S - adenosyl - L - methionine ( SAM ) on the biological behaviors of colorectal cancer ( CRC ) cell lines, SW620 and LoVo. Methods LoVo and SW620 were treated with SAM for 6 days. Cell proliferation and cell cycle were assessed by plate colony formation assay and flow cytometry assay , respectively. Meanwhile, the efficacy of SAM on CRC cell apoptosis was also analyzed by flow cytometry. S - adenosyl -L-homocysteine ( SAH ) -treated cells and mock - treated cells were used as control groups in our study. Results A significant inhibition of cell proliferation was observed in SAM - treated SW620 and LoVo cells as compared with SAH -treated cells ( P ＜0. 001 ). In addition, a significant reduction of the plate colony formation was also observed in SAM-treated cells as compared with SAH - treated cells and mock - treated cells ( P ＜ 0. 001 ). However, no apoptosis peak was detected in SAM - treated cells. Conclusion Methylation agent SAM can not only inhibit cell proliferation but also result in the arrest of cell cycle. Our study suggests a potential role of SAM in the clinical treatment of CRC.关键词
结直肠癌/DNA甲基化/S-腺苷甲硫氨酸/细胞周期Key words
colorectal cancer/ DNA methylation/ S - adenosyl - L - methionine/ cell cycle引用本文复制引用
王博,金贺,李婷婷,林洁,丁彦青..S-腺苷甲硫氨酸对结直肠癌细胞生物学行为的影响[J].广东医学,2011,32(24):3157-3160,4.基金项目
国家自然科学基金资助项目(编号:30800414) （编号:30800414）
