南方医科大学学报2011，Vol.31Issue(11)：1812-1818,7.DOI:44-1627/R.20111027.0841.004
用于活体成像的人三阴性乳腺癌MDA-MB-231细胞系的构建
Establishment of a bioluminescent MDA-MB-231 cell line for in vivo imaging of human triple-negative breast cancer xenograft
摘要
Abstract
Objective To investigate the apoptosis-inducing effect of honokiol on human non-Hodgkin lymphoma Raji cells and the possible mechanism. Methods Raji cells were treated with different concentrations of honokiol, and the proliferation of the cells was detected using MTT assay. Flow cytometry was employed to analyze the cell cycle changes and apoptosis of honokiol-treated cells. Caspase 8 activity in the cells was measured by caspase 8 kit, and RT-PCR was used to detect the expression of apoptosis-related genes Bcl-2, Bad, and Bax. Results Honokiol significantly inhibited the growth of Raji cells in a time- and dose-dependent manner, with IC50 concentration of 17.53, 12.61, and 7.4 μg/ml at 12, 24, 48 h, respectively. Flow cytometry revealed cell cycle arrest at G0/G1 phase following honokiol treatment. The apoptosis rates of Raji cells treated with 7.5 and 15 μg/ml honokiol were significantly higher than that of the control cells [(18.24+2.53)%, (28.44±2.48)% vs (4.84±1.15)%, P<0.01]. Caspase 8 activity in Raji cells was significantly enhanced by honokiol (P<0.05). The mRNA expression of the apoptosis-promoting gene Bad was significantly increased following honokiol treatment (P<0.01), while the expressions of Bcl-2 and Bax remained unchanged. Conclusion Honokiol can induce apoptosis in Raji cells possibly in relation to enhancement of caspase 8 activity and Bad gene expression.关键词
活体生物发光成像/MDA-MB-231细胞/三阴性乳腺癌/裸鼠/移植瘤模型Key words
honokiol/ non-Hodgkin lymphoma/ apoptosis/ caspase 8/ Bcl-2/ Bad/ Bax分类
医药卫生引用本文复制引用
王珂,谢四梅,何建军,任予,夏海滨,张新伟..用于活体成像的人三阴性乳腺癌MDA-MB-231细胞系的构建[J].南方医科大学学报,2011,31(11):1812-1818,7.基金项目
陕西省科学技术研究发展计划项目(2009K12-01) （2009K12-01）
