广东医学2011，Vol.32Issue(23)：3028-3031,4.
隐丹参酮对人胆管癌HCCC-9810细胞中Survivin基因表达的影响
The effects of cryptotanshinone on the expression of survivin in human cholangiocarcinoma cell of HCCC-9810
摘要
Abstract
Objective To investigate the sensitivity of cholangiocarcinoma HCCC-9810 cell lines to cryptotan-shinone; and to evaluate the effects of cryptotanshinone on cell cycle, apoptosis and survivin expression of cholangiocarcinoma cells. Methods HCCC -9810 cell lines were cultured in vitro and treated with cryptotanshinone of different concentrations. The sensitivity of cholangiocarcinoma cell(HCCC-9810)to cryptotanshinone was assessed with cell morphological observation and MTT method. Flow cytometry and RT-PCR were applied for evaluation of cell cycle and survivin mRNA expression, respectively, in HCCC -9810 cells. Results Cryptotanshinone significantly inhibited the proliferation of the HCCC -9810 cells following concentration and time dependent manners. As the optimal treatment spanned, HCCC -9810 cells was caught in G0/G1 phase, with reduced proportion of cells in S phase and increased apoptosis, and the survivin mRNA expression was down-regulated. Conclusion Cryptotanshinone can inhibit the growth of the HCCC-9810 following time and concentration dependent manners, Furthermore, cryptotanshinone induces apoptosis of HCCC-9810 cell, probably via down-regulation of survivin expression and affecting the cell cycle.关键词
人胆管癌细胞(HCCC-9810)/Survivin基因/隐丹参酮Key words
human cholangiocarcinoma cell(HCCC-9810)/survivin gene/cryptotanshinone引用本文复制引用
陈春雷,弓建华,缪辉来,林晖,林俊琼..隐丹参酮对人胆管癌HCCC-9810细胞中Survivin基因表达的影响[J].广东医学,2011,32(23):3028-3031,4.基金项目
广东省建设中医药强省课题基金资助项目(编号:20111090) （编号:20111090）
