中国组织工程研究与临床康复2011，Vol.15Issue(46)：8609-8612,4.DOI:10.3969/j.issn.1673-8225.2011.46.014
人脐静脉内皮细胞单层通透性改变的效应因子
Effective factors for regulating monolayer permeability of human umbilical vein endothelial cells
摘要
Abstract
BACKGROUND: The molecular mechanism underlying exogenous stimulation-caused vascular barrier dysfunction remains poorly understood in the field of vascular pathophysiology.OBJECTIVE: To investigate the effective factors for regulating monolayer permeability of human umbilical vein endotielialcelfe and search for the effective treatment target.METHODS: Human umbilical vein endothelial cells (HUVECs) were exposed to lipoporysaccharide (LPS) with and without s p ecrf I c I nh ib rtors of R hoA and F-a ctin stress f ibr e. C hanges in RhoA/SR F/F- actin actmily in ass ociation wirth ce II pe rme ability arid F-actin rearrangement signaling were investigated by immunostaining. Transvuell assay andvuestem blot anarysis. RESULTS AND CONCLUSIONS: When HUVECs were treated with LPS, F-actin cytoskeleton rearrangement was detected by rhodamine-phalloidin staining in a dose and time-dependent manner, meanwhile the HUVEC hyperpermeabilrty was investigated. Furthermore, the RhoA activity and SRF trans localization into nuclei were identified to be associated with elevation of permeability in HUVECs. The effects were blocked when cells were pretreated with Y27632 or Latrunculin B. Respectively. Moreover, Latrunculin B added also inhibited the trans localization of SRF into the nuclei mediated by RhoA activity. Our resub have identified the RhoA/SRF and their substrates F-actin rearrangement mediated LPS-induced changes in HUVECs permeability.关键词
人脐静脉内皮细胞/通透性/细胞骨架蛋白/RhoA/ERK分类
医药卫生引用本文复制引用
闫承慧,于海波,张效林,康建,韩雅玲..人脐静脉内皮细胞单层通透性改变的效应因子[J].中国组织工程研究与临床康复,2011,15(46):8609-8612,4.基金项目
国家自然科学基金面上项目(30770793,30971218,81070097) （30770793,30971218,81070097）
国家自然科学基金青年基金项目(30800465)资助 （30800465）
辽宁省自然科学基金(20092088)和辽宁省科技攻关项目(2009225009-9) 资助. （20092088）
