中国组织工程研究与临床康复2011，Vol.15Issue(46)：8591-8594,4.DOI:10.3969/j.issn.1673-8225.2011.46.010
低氧条件下成骨细胞的增殖与分化
Effects of hypoxia on proliferation and differentiation of rat osteoblasts
摘要
Abstract
BACKGROUND: Hypoxia influences bone metabolism by many means and exhibits negative effects on bone generation andbone healing.OBJECTIVE: To explore the effects of hypoxia on proliferation and differentiation of rat osteo blasts and investigate theundeTrying mechanisms.METHODS: Primary rat osteoblasts were isolated from exceed calvarial bones of neonatal mice and cultured in vitro. Thesecond passage of osteoblasts were cultured under hypoxic(3% Oz) and normoxic(20% Oz) conditions.RESULTS AND CONCLUSION: The proliferation levels, akaline phosphatase actwrties. Oste oca Icin contents and the number ofcalcium nodules in the hypoxic group were significantly lower compared with the normoxic group (P< 0.05 or (P <0.01). It meansthat hypoxia inhibits the proliferation, differentiation and functions of rat osteoblasts. The mRNA expression of bonemorphogenetic protein-2 and Runx2 in osteoblasts cultured in 3% Oz was louver than that cultured in 20% Oz (P< 0.05 orP <0.01). It means that the mRNA expression of Runx2 and bone morphogenetic protein-2 was decreased in hypoxic conditions.These findings suggest that hypoxia can inhibit the proliferation and differential) on of rat osteoblasts through inhibiting Runx2 andBMP-2 mRNA expression in vit/v.关键词
低氧/成骨细胞/增殖/分化/Runx2/骨形成发生蛋白2分类
医药卫生引用本文复制引用
高文魁,王德元,李智钢,闫自强,白峰,王伟..低氧条件下成骨细胞的增殖与分化[J].中国组织工程研究与临床康复,2011,15(46):8591-8594,4.基金项目
青海省科学技术厅科技创新能力促进计划项目(2010.Z.744),课题名称:低氧环境在骨质疏松发生发展过程中的作用机制研究. （2010.Z.744）
