中国组织工程研究与临床康复2011，Vol.15Issue(46)：8603-8608,6.DOI:10.3969/j.issn.1673-8225.2011.46.013
apelin-13下调caveolae可促进血管平滑肌细胞增殖
apelin-13 promotes proliferation of vascular smooth muscle cells by downregulating caveolae expression
摘要
Abstract
BACKGROUND: Previous results have shown that caveolae is likely to participate in a pel in-13 promotion of vascular smoothmuscle cell proliferation.OBJECTIVE: APJ e a G-protein coupled receptor, and rts ligand is apelin peptide. Previously, we reported PI3K and ERK1Ssignal pathway mediated proliferation of vas cular smooth muscle cells (VSMCs) induced by apelin-13. ThEstudy e to observecaveolae invoked in rat VSMCs proliferation induced by apelin-13 and to determine whether caveolin-1 proteh binds to signalmolecules PI3K, ERK1/2to mediate VSMC proliferation induced by apelin-13.METHODS: VSMCs were prepared from male Sprague-Dawley ratthoracic aorta by the primary-expl ant method. The effect of &cyclodextrin on cell proliferation induced by apelin-13 was measured by MTT assay. VSMCs were incubated with apel'n-13 andB-cyclo dextrin (B-CD), caveolir-1 expression was detected by western blot assay. Formation of high molecular weight poryp rote incomponent including caveolin-1 and PI3K /ERK1/2 was detected by immunoprecipitation.RESULT SAND CONCLUSION: 6-CD(5 mmol/L, 25 hours) significantly enhanced VSMCs proliferation induced by apelin-13(P < 0.05). Treating VSMCs with apelin-13 (0, 1, 2, 4, 8 u mol/L) downregulated apelin-13-induced expression of caveoln-1 andthe effect was distinct at 1μ mol/L<p < 0.05). Pretreatment of the celb with 5 mmoVL B-CD could strength the do wnregulation ofapelin-13-induced caveolin-1 expression (p < 0.05). Apelin-13 may induce dissociation of PI3K(ERK1/2) wrth caveolin-1 inVSMCs compared with the control group. Thesefindings suggest that caveolae t> invoked in apelin-13-induced VSMCsproliferation.关键词
血管平滑肌细胞/apelin-13/APJ/caveolae/caveolin-1/β-环糊精/PI3K/ERK1/2分类
医药卫生引用本文复制引用
毛小环,李兰芳,高晶,杨莉,柳威,秦旭平,陈临溪..apelin-13下调caveolae可促进血管平滑肌细胞增殖[J].中国组织工程研究与临床康复,2011,15(46):8603-8608,6.基金项目
国家自然科学基金项目(30901577) 和教育部留学回国人员科研启动基金(20091590). （30901577）
