中国组织工程研究与临床康复2011，Vol.15Issue(46)：8706-8711,6.DOI:10.3969/j.issn.1673-8225.2011.46.037
川芎嗪可影响肿瘤坏死因子a刺激肝星状细胞结缔组织生长因子、核因子κB及相关基因产物的表达
Effects of tetramethylpyrazine on expression of connective tissue growth factor, nuclear factor kappa B and its related gene products in tumor necrosis factor-alpha stimulated hepatic stellate cells
摘要
Abstract
BACKGROUND: Previous studies have shown that tetramethylpyrazine (TMP) can decrease expression of connective tissue growth factor (CTGF), nuclear factor kappa B (NF-?B) and delay the formation of hepatic fibrosis. But the mechanism and the correlation of CTGF and NF-?B was not clear.OBJECTIVE: To investigate the effects of TMP on expression of CTGF, NF-?B and its related gene product interleukin-6(IL-6) in tumor necrosis factor-alpha (TNF-a) stimulated hepatic stellate cells.METHODS: Hepatic stellate cell-T6 (HSC-T6) was cultured to logarithmic growth phase for experiments. Cells were divided into four groups: control group: only cells were added; TNF-a group: 10 ug/L TNF-a was added; TMP intervention group: pretreated with 10 μg/L TNF-a for 30 minutes and then 50,100, 200, 400, 600, 1 000 mg/L TMP was added; PDTC group: pretreated with 10 μ g/L TNF-a for 30 minutes, and then stimulated with 18 u mol/L PDTC, a blocker of NF-?B.RESULTS AND CONCLUSIONS: MTT assay results showed that TMP inhibited the proliferation of HSC-T6 at a concentration of 100, 200, 400, 600, and 1 000 mg/L in a concentration dependent manner (P <0.05). Immunocytochemical staining and western blot analysis showed that 200, 400, 600 mg/L TMP and 18 u mol/L PDTC could decrease the expression of TNF-ainduced CTGF, NF-?B and IL-6 (P < 0.01) in HSC-T6 cells and with increasing concentrations of TMP, the inhibitory effects were enhanced, and PDTC could inhibit their expression effectively. Correlation analysis showed that the expression of cTGF and NF-?B in HSCT6 cells was positively correlated (r=0.980, P < 0.01).These data indicated that TMP could inhibit the expression of cTGF, NF-?Band IL-6 in hepatic stellate cells and inhibit the proliferation of hepatic stellate cells.关键词
川芎嗪/肿瘤坏死因子a/肝星状细胞/结缔组织生长因子/核因子kB分类
医药卫生引用本文复制引用
李艳芳,李孝生..川芎嗪可影响肿瘤坏死因子a刺激肝星状细胞结缔组织生长因子、核因子κB及相关基因产物的表达[J].中国组织工程研究与临床康复,2011,15(46):8706-8711,6.基金项目
重庆市科委自然科学基金项目项目(2006BB5428),课题名称:川芎嗪对HSC的影响及CTGF 信号转导的调控机制探讨. （2006BB5428）
