南方医科大学学报2011，Vol.31Issue(11)：1840-1845,6.DOI:44-1627/R.20111101.1617.001
转化生长因子β1及其信号转导阻滞对系统性硬化症皮损中成纤维细胞转分化的影响
Transdifferentiation of fibroblasts into myofibroblasts in the skin lesion of systemic sclerosis: role of transforming growth factor β1 and its signal transduction
摘要
Abstract
Objective To observe the effect of pioglitazone on hypoxia/reoxygenation injury and the expression of protein kinase C (PKC) in neonatal rat cardiomyocytes. Methods Neonatal Sprague-Dawley rat cardiomyocytes in primary culture were treated with pioglitazone or GW9662 for 24 h prior to hypoxia/reoxygenation injury. Cardiomyocyte apoptosis was evaluated with Hoechst33258 staining and the expression of PKC was detected using Western blotting. Results In the early stage of hypoxia/reoxygenation injury, the apoptosis rates of the cardiomyocytes increased significantly from (0.20±0.03)% of the control level to (12.22±1.45)% (P<0.05). Pretreatment with pioglitazone significantly lowered the apoptosis rate of the cardiomyocytes with hypoxia/reoxygenation injury to (8.32±0.89)%, and this effect was antagonized by GW9662, a specific blocker of peroxisome proliferators activated receptors γ (PPARγ). Pioglitazone did not cause increased expression of PKC in the cardiomyocytes. Conclusion Pioglitazone can ameliorate neonatal rat cardiomyocyte injury induced by hypoxia/reoxygenation partially by activating PPARγ and does not increase the expression of PKC in the cells.关键词
系统性硬化症/α-平滑肌肌动蛋白/肌成纤维细胞/转化生长因子-β1/信号转导Key words
peroxisome proliferators activated receptors gamma/ ischemia-reperfusion injury/ ischemic preconditioning分类
医药卫生引用本文复制引用
刘彤,胡晓丁..转化生长因子β1及其信号转导阻滞对系统性硬化症皮损中成纤维细胞转分化的影响[J].南方医科大学学报,2011,31(11):1840-1845,6.基金项目
陕西省科技攻关项目(2008K15-06) （2008K15-06）
