南方医科大学学报2011，Vol.31Issue(11)：1846-1850,5.DOI:44-1627/R.20111107.0927.007
肠道病毒71型VP1～VP4基因克隆及其表达产物的免疫原性
Gene cloning and immunogenicity analysis of the structural proteins VP1-VP4 of enterovirus 71
摘要
Abstract
Objective To investigate the changes of auditory parameters during anesthesia and establish the assessment indicators for anesthesia monitoring in animal experiments. Methods BALB/c mice of 4 to 6 weeks were given a single intraperitoneal dose of urethane, and the auditory evoked potential in the surgically exposed inferior colliculus in response to pure tone stimulation was recorded during urethane metabolism. The latency and amplitude data of the waves were extracted using Matlab software to analyze their variations during urethane metabolism. Results The latency of the auditory evoked potential showed slight variation and was well correlated to time. The latency decreased progressively during urethane metabolism, fast in the initial 2 h and tending to stabilize afterwards. Conclusion The latency of the auditory evoked potential can be more suitable indicators than the amplitude for anesthetic effect monitoring.关键词
肠道病毒71型/基因克隆/原核表达/免疫原性Key words
anesthetic effect monitoring/ auditory evoked potential/ urethane分类
医药卫生引用本文复制引用
宋远斌,余楠,何思杰,陈欣欣,王斌,车小燕,曾其毅..肠道病毒71型VP1～VP4基因克隆及其表达产物的免疫原性[J].南方医科大学学报,2011,31(11):1846-1850,5.基金项目
国家重大科技专项课题(2009ZX10004-306) （2009ZX10004-306）
广州市医药卫生科技重大项目(201102A211007) （201102A211007）
