中国农业科学2011，Vol.44Issue(14)：3053-3059,7.DOI:10.3864/j.issn.0578-1752.2011.14.023
甘蓝抗枯萎病SCAR标记的开发
Development of A SCAR Marker for Fusarium Wilt Resistance in Cabbage
摘要
Abstract
[ Objective 1 The objective of the experiment is to develop a SCAR(sequence characterized amplified region) marker of Fusarium wilt resistant gene FOC-1 in cabbage. [Method] Bulked segregation analysis (BSA) DNApools were constructed by F2 individuals derived from the cross between resistant '8024'X susceptible '6A' cross. An AFLP marker genetically linked to the Fusarium wilt resistant gene was identified and it was converted into a more valuable and simple SCAR marker S46M48i99 consequently. Genetic linkage between S46M48199 and FOC-1 was analyzed by using 142 F2 progenies. The validity and practicality of S46M48i99was verified in two different cabbage F2 populations F66 and Cl. [Result] One stable SCAR marker S46M48,99 linked in repulsion to the dominant resistant allele at 2.78 cM from the FOC-1 locus was developed successfully. The validity and practicality of S46M48]99 in two other populations revealed significant consistency with phenotype assay (81% and 83%, respectively). [Conclusion] The SCAR marker developed in this study could be used for MAS of Fusarium wilt resistance in cabbage.关键词
甘蓝/枯萎病/抗性/AFLP/SCARKey words
Brassica oleracea var/capitata L./Fusarium wilt/disease resistance/AFLP/SCAR引用本文复制引用
姜明,赵越,颉建明,田仁鹏,陈延阳,康俊根..甘蓝抗枯萎病SCAR标记的开发[J].中国农业科学,2011,44(14):3053-3059,7.基金项目
国家"十一五"科技支撑计划项目(2008BADB1B02,2009BADB8B03)、国家"十二五"科技支撑计划项目(2011BAD35B07)、北京市自然科学基金项目(6102012)、北京市农林科学院常规育种财政专项、北京市优秀人才项目、北京市农林科学院青年科研基金项目 （2008BADB1B02,2009BADB8B03）
