分析化学2011，Vol.39Issue(8)：1134-1140,7.DOI:10.3724/SP.J.1096.2011.01134
生物基质中全氟辛酸与全氟辛烷磺酸及其前体化合物的超高效液相色谱-串联质谱法研究
Investigation of Perfluorooctanoic Acid and Perfluorooctanesulfonate and Their Precursors in Biological Matrix by Ultra Performance Liquid Chromatography and Tandem Mass Spectrometry
摘要
Abstract
An original method based on ultra performance liquid chromatography tandem mass spec-trometry (UPLC-MS/MS) was developed for the determination of perfluorooctanoic acid (PFOA), perfluorooctanesulfonate (PFOS) and their precursors in biological matrix. Target compounds were extracted with water or methanol in corresponding biological matrix by ultrasonic. Then the extract was purified by solid phase extraction (Waters Oasis(R) WAX 6cc), after that the precursors com-pounds were eluted with methanol and PFOA and PFOS were eluted with 9% NH4OH in methanol. Finally the target compounds were separated on a Waters ACQUITYTM BEH C18 column (50 mm× 2. 1 mm ×1. 7 μm) and detected by negative electrospray ionization mass spectrometry in multiple re-action monitoring mode. The average recoveries for four target compounds in two biological matrixs were 94. 6% -128. 4% by using internal standard method and the relative standard deviations were 2.5% -14. 9%. For the four target compounds in two biological matrixs, the limits of detection of the method were in the range of 0. 01 - 0. 42 μg/L and the limits of quantification were in the range of 0. 04-1. 5 μg/L. With high accuracy and sensitivity, the method was simple and rapid, and can apply to evaluate the metabolic characterization and the source of exposure for PFOA and PFOS in biological matrix.关键词
超高效液相色谱-串联三重四级杆质谱(UPLC-MS/MS)/生物基质/固相萃取/全氟辛酸/全氟辛烷磺酸/前体化合物Key words
Ultra performance liquid chromatography and tandem mass spectrometry/ Biological matrix/ Solid phase extraction/ Perfluorooctanoic acid/ Perfluorooctanesulfonate/ Precursors compounds引用本文复制引用
王雨昕,李敬光,赵云峰,郭斐斐,吴永宁..生物基质中全氟辛酸与全氟辛烷磺酸及其前体化合物的超高效液相色谱-串联质谱法研究[J].分析化学,2011,39(8):1134-1140,7.基金项目
本文系国家自然科学基金项目(Nos.21077098,20607021)资助 （Nos.21077098,20607021）
