分析化学2011，Vol.39Issue(8)：1166-1170,5.DOI:10.3724/SP.J.1096.2011.01166
质谱特征结合等效链长定性分析植物油中的脂肪酸
Identification of Fatty Acids in Vegetable Oils by Mass Spectrometry and Equivalent Chain Length
摘要
Abstract
To automatically identify fatty acids in vegetable oils, a method was proposed using mass spectrometry and equivalent chain length (ECL). According to mass spectral characteristics, the type of fatty acids in the sample and its saturated fatty acid methyl esters were identified, then the reten-tion time of saturated fatty acid methyl esters was used to calculate the ECL value of the unsaturated ones, and the ECL values of the interest were compared to the database established in our lab to indentify the molecular structure of all the components in the samples. The fatty acids of five vegetable oils were extracted and esterified by NaOH-CH3OH, and subsequently analyzed by GC/ MS. Sufficient separation for the homologous and isomers of fatty acid methyl esters was obtained with DB-23(30 m×0. 25 mm×0. 25 μrn) capillary column. The result indicated that unsaturated fatty acids extracted from the five vegetable oils were different in chemical composition and relative con-tent. Using this method, contents of homologues and different isomers of fatty acid methyl esters can be automatically determined without standards. The method can be applied to the investigation of the compositions of fatty acids in lipid and food samples.关键词
脂肪酸/质谱特征/等效链长(ECL)/植物油/气相色谱/质谱Key words
Fatty acid/ Mass spectral characteristics/ Equivalent chain length/ Vegetable oils/ Gas chromatography/mass spectrometry引用本文复制引用
梁楠楠,张良晓,王向利,谭斌斌,梁逸曾..质谱特征结合等效链长定性分析植物油中的脂肪酸[J].分析化学,2011,39(8):1166-1170,5.基金项目
本文系国家自然科学基金项目(Nos.20875104,21075138)资助 （Nos.20875104,21075138）
