分析化学2011，Vol.39Issue(8)：1171-1175,5.DOI:10.3724/SP.J.1096.2011.01171
基于金纳米棒-辣根过氧化物酶协同效应的过氧化苯甲酞生物传感器
A Novel Benzoyl Peroxide Biosensor Based on the Synergistic Effects of Gold Nanorods and Horseradish Peroxidase Composite Membrane
摘要
Abstract
A novel benzoyl peroxide (BPO) biosensor was prepared by self-assembling of gold nano-rods (AuNRs) and horseradish peroxidase (HRP) on the surface of gold electrode by layer-by-layer. The electrocatalytic behavior and the optimum conditions of the biosensor were investigated by cyclic voltammetry and I-t curves in PBS. Taking the advantages of synergistic effect between AuNRs and HRP, the performance of the biosensor has been improved remarkably. Under the optimum condi-tions, -0. 02 V and PBS pH 7, the current response of the biosensor was linearly proportional to BPO concentration ranged from 5.0× 10-6 mol/L to 1.0×10-4 mol/L with a correlation coefficient of 0. 9993 for benzoyl peroxide determination, and a detection limit of 8. 5×10-7 mol/L. The linear regression equation was I(μA) = 12. 6796C(mmol/L)+0. 2406. The recovery of the BPO in flour and the RSD were 97. 9% - 100. 1% and 0. 5% - 2. 1% with 6 times determinations, respectively. The method was applied to the determination of BPO in flour in the market with satisfactory results.关键词
过氧化苯甲酰/金纳米棒/辣根过氧化物酶/生物传感器/协同效应/面粉Key words
Benzoyl peroxide biosensor/ Gold nanorods/ Horseradish peroxidase/ Biosensor / Syner-gistic effect / Flour引用本文复制引用
曾云龙,李霞飞,黄昊文,唐春然,赵环,胡娅梅,李进..基于金纳米棒-辣根过氧化物酶协同效应的过氧化苯甲酞生物传感器[J].分析化学,2011,39(8):1171-1175,5.基金项目
本文系国家白然科学基金(No.21075035)、湖南大学化学生物传感与化学计量国家重点实验室基金(No.2008006),湖南省自然科学基金(No.07JJ6027)和湖南省高校创新平台开放基金(No.10k024)资助项目 （No.21075035）
