分析化学2011，Vol.39Issue(8)：1181-1185,5.DOI:10.3724/SP.J.1096.2011.01181
基于葡萄糖/葡萄糖氧化酶/H2O2/L-酪氨酸/辣根过氧化物酶反应体系的荧光毛细分析法测定血糖
Determination of Blood Glucose by Fluorescence Capillary Analysis Based on Glucose/Glucose Oxidase/HZOZ/L-tyrosine/Horseradish Peroxidase Reaction System
摘要
Abstract
A novel fluorescent reaction system of glucose/glucose oxidase (GOD)/hydrogen peroxide/ L-tyrosine/horseradish peroxidase (HRP) combined with a fluorescence capillary analysis (FCA) was established for developing a sensitive glucose enzyme FCA (GE-FCA). After studying, the new reac-tion, the optimum conditions were as follows: concentrations of GOD and HRP were both 2000 U/L, concentration of L-tyrosine was 1. 0 mmol/L, pH of phosphate buffer solution was 8. 5, reaction tem-perature was 35 ℃ and reaction time was 20 min. The GE-FCA's linear range was 5. 0-140 μmol/L, relative standard deviation was less than 4. 0% and detection limit was 2. 65 μmol/L. The method has been successfully applied for the determination of blood glucose and the recovery was in the range of 98%-104%. Compared with routine methods, merits of the GE-FCA was simple in operation, less in sample volume and dosage of chemical as well as enzymic reagents (only 9 μL), wide in linear range, strong in anti- interference ability and low cost, it will be easily popularized.关键词
荧光毛细分析法/葡萄糖氧化酶/辣根过氧化物酶/血糖Key words
Fluorescence capillary analysis/ Glucosse oxidase/ Horseradish peroxidase/ Blood glucose引用本文复制引用
李乔婧,高秀峰,杜运动,陈婷梅,李永生..基于葡萄糖/葡萄糖氧化酶/H2O2/L-酪氨酸/辣根过氧化物酶反应体系的荧光毛细分析法测定血糖[J].分析化学,2011,39(8):1181-1185,5.基金项目
本文系四川大学214人才引进振兴计划基金项目(No.0082204127067)和上海棱光技术公司资金共同资助 （No.0082204127067）
