分析化学2011，Vol.39Issue(8)：1251-1255,5.DOI:10.3724/SP.J.1096.2011.01251
以离子液体为流动相的高效液相色谱-间接紫外检测法分析四甲基按根离子
Analysis of Tetramethyl Ammonium Ion by High Performance Liquid Chromatography-Indirect Ultraviolet Detection Using Ionic Liquids as Mobile Phase
摘要
Abstract
A method was developed for the determination of tetramethyl ammonium ion by reversed phase high performance liquid chromatography with indirect ultraviolet (UV) detection. Chromato-graphic separation was performed on a ZORBAX ODS reversed phase column using ionic liquids + organic solvents (acetonitrile or methanol) as mobile phase. Chromatographic detection was carried out by indirect UV detection. The effects of the ultraviolet detection wavelength and mobile phases on the separation and determination of tetramethyl ammonium ion were investigated. The optimized chromatographic conditions for the determination of tetramethyl ammonium ion were as follows: 80% 1-ethyl-3-methyl-imidazolium tetrafluoroborate aqueous solution (0. 5 mmol/L, pH 3. 5 adjusted with acetic acid) + 20% acetonitrile as mobile phase, detection wavelength 210 nm, column temperature 30 °C , flow rate 1. 0 mL/min. Under these conditions, the detection limit (S/N=3) of tetramethyl ammonium ion was 2. 60 mg/L. The relative standard deviations (RSD) of the chromatographic retention time and peak areas for tetramethyl ammonium ion were 0. 04% and 0. 3% (n = 5) , respec-tively. The calibration curve showed good linearity over the tested range from 10 to 204 mg/L for tetramethyl ammonium ion concentration with the correlation coefficients of 0. 9999. The method has been applied to the determination of tetramethyl ammonium ion in ionic liquid tetramethyl ammonium bromide. The recovery of tetramethyl ammonium ion after spiking was 99. 3%.关键词
高效液相色谱/间接紫外检测/四甲基铵根离子/离子液体Key words
High performance liquid chromatography/ Indirect ultraviolet detection/ Tetramethyl ammonium ion/ Ionic liquid引用本文复制引用
董影杰,于泓,王婧菲,黄旭..以离子液体为流动相的高效液相色谱-间接紫外检测法分析四甲基按根离子[J].分析化学,2011,39(8):1251-1255,5.基金项目
本文系黑龙江省自然科学基金(No.B200909)和黑龙江省教育厅科学技术研究项目(No.11541088)资助 （No.B200909）
