高等学校化学学报2011，Vol.32Issue(7)：1622-1628,7.
肾上腺髓质素微球复合PLGA/纳米轻基磷灰石支架材料的制备及生物活性评价
Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin
摘要
Abstract
By tripolyphosphate ( TPP) as cross-linker, chitosan microspheres ( CMs) loaded with ad-renomedullin( ADM) were prepared by an emulsion-ionic cross-linking method. Then, porous poly(lactide-co-glycolide) ( PLGA)/nano-hydroxyapatite ( nHA)/CMs scaffold was developed by thermally induced phase separation. Scanning electron microscope was used to analyze the characterization of the microspheres and the scaffold and the drug release from them was tested in vitro. Also, the hemolytic effect of the scaffold was tested to evaluate the toxicity of the biomaterial. MG63 cell line and HUVEC cell line were cultured for testing the bioactivity of the scaffold loading ADM. After the cells adhered on the scaffold, the cell proliferation and differentiation were analyzed by methyl thiazolyl tetrazolium(MTT) assay, fluorescent staining and alkaline phos-phatase(ALP) assay. The results show that the diameter of the microspheres is well-distributed and the PLGA/nHA/CMs scaffold shows a typical microstructure of polymeric foam. The pores of the scaffold ranged from 30 μm to 220 μm and they were interconnected. The hemolytic rate of the biomaterial was less than 5% , which was in line with national standard of medical materials. This study confirmed that PLGA/nHA/CMs/ ADM and PLGA/nHA/CMs scaffold could accelerate the proliferation of osteoblast-like cells and vascular endothelial cells and also could stimulate the differentiation of osteoblast-like cells.关键词
壳聚糖/微球/聚丙交酯-乙交酯/纳米羟基磷灰石/肾上腺素质素Key words
Chitosan/Microsphere/Poly(lactide-co-glycolide)/Nano-hydroxyapatite/Adrenomedullin分类
化学化工引用本文复制引用
王林,李春艳,何盼,付丽,周延民,陈学思..肾上腺髓质素微球复合PLGA/纳米轻基磷灰石支架材料的制备及生物活性评价[J].高等学校化学学报,2011,32(7):1622-1628,7.基金项目
口腔疾病研究国家重点实验室创新人才培养课题基金(批准号:5KLODd2009001)及吉林省产业技术研究与开发项目(批准号:20101935)资助. （批准号:5KLODd2009001）
