高等学校化学学报2011，Vol.32Issue(7)：1492-1496,5.
Pd( Ⅱ)与色氨酸、酪氨酸及苯丙氨酸相互作用的荧光光谱
Fluorescence Spectroscopy Study on the Interaction Between Pd(Ⅱ)and Tryptophan,Tyrosine and Phenylalanine
摘要
Abstract
The fluorescence of three aromatic amino acids, tryptophan(Tip) , tyrosine(Tyr) and phenylala-nine(Phe) could be quenched by Pd( E ). The changes of absorption spectra, the effect of temperature and the quenching constant ￡5V indicated this procedure was static quenching, which was due to the formation of ground-state complex between Pd( II ) and the amino acids. The results showed that in the presence of certain concentration of Cl- , Pd( II ) coordinated with amino acids by N-N and N-O to form mixed-type ternary complexes Pd(HR)Cl2 (Trp and Phe systems) and Pd( H2R) Cl2 (Tyr system), respectively. This fluorescence system not only can be used in the study on the interaction between Pd ( II ) and aromatic amino acids, but also creates conditions for determining Pd( II ) by amino acid (especially Trp) probe as a sensitive fluorescence quenching method.关键词
荧光猝灭/Pd(Ⅱ)/色氨酸/酪氨酸/苯丙氨酸Key words
Fluorescence quenching/Pd( II )/Tryptophan/Tyrosine/Phenylalanine分类
化学化工引用本文复制引用
许倩影,刘忠芳,胡小莉,孔玲,刘绍璞..Pd( Ⅱ)与色氨酸、酪氨酸及苯丙氨酸相互作用的荧光光谱[J].高等学校化学学报,2011,32(7):1492-1496,5.基金项目
国家自然科学基金(批准号:20875078)资助. （批准号:20875078）
