干旱地区农业研究2011，Vol.29Issue(4)：223-227,5.
荒漠植物柠条根瘤菌的抗逆性及其系统发育分析
Analysis of stress resistance and phylogenesis of rhizobia isolated from Caragsana spp.
摘要
Abstract
Several biochemical characteristics of 62 rhizobia isolated from Caragsana spp . In Baijitan National Na-ture Reserve in Ninaxia were detected , including resistance to salt , acid-alkali, temperature variation and intrinsic an tibiotics . The results indicated that 70 .8% strains could tolerate NaCl stress at 4% concentration . All of the strains could tolerate Amp stress at 100 μg/mL as well as could grow at a range of pH from 5~11. Strains CNXE4, CNXLW24 and CNXC3 had high resistance , they could grow at pH from 4~ 12 ,could tolerate 6% concentration NaCl, 50 μg/mL KM and SM , 300 μg/mL AMP , and could grow after being exposed to 60°C for 10 min . The methods of 16S rDNA PCR RFLP and 16S rDNA sequencing were used to analysis the diversity and phylogenesis of 62 rhizobia strains . The results revealed that all the tested strains belonged to the genus of Rhizobium , Sinorhizobium , Agrobacterium , Phyllobacterium and Brady rhizobium . The rhizobia isolated from Caragsana spp . Showed a rich genetic diversity .关键词
柠条/根瘤菌/抗逆性/系统发育Key words
Caragsana spp ./ rhizobia/stress resistance/phylogenesis分类
农业科技引用本文复制引用
代金霞,王玉炯,郭晶静,武雪娟,张晓玲..荒漠植物柠条根瘤菌的抗逆性及其系统发育分析[J].干旱地区农业研究,2011,29(4):223-227,5.基金项目
宁夏自然科学基金资助项目(NZ1040) （NZ1040）
