摘要
Abstract
Objective To study the bcl-2 mRNA expression in bone marrow mononuclear cells of childhood B-ALL, investigate the effect of bcl-2-ASODN on proliferation, apoptosis and sensitivity to chemotherapy of childhood B-ALL cells in vitro, speculate the role of bcl-2 in the mechanism, development and prognosis of childhood B-ALL, and make a perspective on the clinical application of ASODN in the future. Methods Bone marrow mononuclear cells of childhood B-ALL were used. The expression of bcl-2 was detected by Real-time Quantity PCR. Bcl-2-ASODN was transferred into cells mediated with lipofectin. Twenty-four hours later, detect the expression of bcl-2 by Real-time Quantity PCR, proliferation of leukemia cell by MIT assay and, cell apoptosis with flowcytometry. Then add chemotherapy drugs to study the chemotherapy sensitvity of leukemia cells. Results The results showed that the bcl-2 mRNA expression in childhood B-ALL was higher than control, especially in chemotherapy resistant group, and the bcl-2 mRNA expression was down regulated by chemotherapy in sensitive group. Bcl-2-ASODN was able to down-regulate bcl-2 mRNA expression, induce apoptosis of B-ALL cells, and enhance the effect of chemotherapy to inhibit cell growth. Conclusion Bcl-2 pLays an important role in childhood B-ALL. The sensitivity of childhood B-ALL cells to chemotherapy is enhanced by hcl-2-ASODN, which suggests that inner bcl-2 in leukemia cells can bring about side effect of drug resistance.关键词
B细胞淋巴瘤/白血病-2基因/儿童急性B淋巴细胞白血病/实时荧光定量/反义寡核苷酸技术Key words
bcl-2/ childhood B-ALL/ real-time quantitative, PCR/ antisense phosphrothioate oliogdeoxynucleotides, ASODN分类
医药卫生
