安徽农业科学2011，Vol.39Issue(20)：12213-12215,3.
miRNA-181b在牛腺垂体中的表达规律及其调控作用研究
Study on the Expression Regulation and Regulatory Functions of miRNA-181b in the Anterior Pituitary of Cattle
摘要
Abstract
[Objective] The research aimed to discuss the differential expression quantity of miRNA-181b in mature (18-month-old) and immature (one- month-old ) cattle's anterior pituitary and its regulation function. [ Method] cDNA library of miRNA in mature ( 18-month-old) and immature (one-month-old) catties anterior pituitary were established. After Solexa high-throughput sequencing of miRNA in the cDNA library, miRNA in anterior pituitary of bulls was identified. miRNA-181b with differential expression were selected from the sequencing results. By real-time quantitative RT-PCR, the expression regulation of miRNA-181 b in the anterior pituitary of Yanbian Cattle in different growth period was validated. And the target genes of miRNA -181b were forecast by using TargetScanS. [ Result] The expression quantity of miRNA-181b had great difference in catties anterior pituitary different growth periods. The expression quantity of miRNA -181b in anterior pituitary of one-month-old cattle was 4.05 times as that in 18-month-old cattle. The binding of miRNA-181b with 838 - 844 bases in 3'untranslated region of FSHβ gene was specific and the binding base sites were UGAAUGUA. [Conclusion] This experiment provided the theoretical basis for the transcription regulation research of FSHβ.关键词
miRNA/实时荧光定量PCR/差异表达Key words
miRNA/ Real-time quantitative RT-PCR/ Differential expression分类
农业科技引用本文复制引用
许橙,张嘉保,袁宝,薛文志,赵茂鑫,陈信,刘颖,于汪洋,许艳丽,马腾壑,高妍..miRNA-181b在牛腺垂体中的表达规律及其调控作用研究[J].安徽农业科学,2011,39(20):12213-12215,3.基金项目
国家自然基金项目(30972100) （30972100）
吉林省科技发展计划项目(201101015). （201101015）
