安徽农业科学2011，Vol.39Issue(20)：12079-12081,12085,4.
车前子乙酸乙酯部位化学成分HPLC指纹图谱研究
Study on the HPLC Fingerprint of Chemical Constituents in Ethyl Acetate Parts of SEMEN PLANTAGINLS
摘要
Abstract
[Objective] To establish the fingerprint of chemical constituents of ethyl acetate parts by HPLC ,so as to provide a basis for quality control and variety identification of SEMEN PLANTAGINIS. [ Method] The chromatographic separation was performed on a,Cl8 column (.250 mm x4.6 mm,5 μm) using the mixture of acetonitrile -0.5% acetic acid aqueous solution as mobile phase in gradient elution. The flow rate was 1.0 ml/min,and the detection wavelength was 270 nm. [ Result] There were 12 common peaks in the HPLC fingerprint of chemical constituents of ethyl acetate parts of 10 batches of SEMEN PLANTAGINI produced in Jiangxi authentic regions. Fingerprint similarity of ethyl acetate parts of SEMEN PLANTAGINI varied obviously for different varieties. [Conclusion] The HPLC fingerprint method was simple,rapid and feasible in the quality control and varieties identification of SEMEN PLANTAGINI.关键词
车前子(SEMEN/PLANTAGINIS)/指纹图谱/质量控制/品种鉴定/乙酸乙酯部位Key words
SEMEN PLANTAGINIS/ Fingerprint/ Quality control/ Variety identification/ Ethyl acetate fraction分类
农业科技引用本文复制引用
朱继孝,王晓云,朱玉野,曾金祥,董丽华,吴波,胡生福,黄碧涛,罗光明,王劭华..车前子乙酸乙酯部位化学成分HPLC指纹图谱研究[J].安徽农业科学,2011,39(20):12079-12081,12085,4.基金项目
国家十一五科技攻关项目(2006BAI06A11-05) （2006BAI06A11-05）
国家科技重大专项子课题(2009ZX09308-002) （2009ZX09308-002）
江西省卫生厅基金项目(2008A061) （2008A061）
江西省教育厅基金项目(GJJ08331). （GJJ08331）
