南京林业大学学报（自然科学版）2011，Vol.35Issue(5)：125-128,4.DOI:10.3969/j.issn.1000-2006.2011.05.028
油茶花药离体培养影响因子研究
Study of impact factor on the anther culture in vitro of Camellia oleifera
摘要
Abstract
The anther of Camellia yuhsiensis were selected as material. The effects of different culture conditions, including different developing period of pollen, time of low temperature pretreatment, and kinds of additives, on inducing callus were investigated. Then we further studied the influence of different plant growth regulators and ratio on callus proliferation. As a result, the best developing stage of pollen on inducing callus from oil-tea anther is tetrad stage; the time of low temperature pretreatment should be below 10 days; and the best medium additives is AgN03, with the high induction rate of 81. 02 %. In the culture of callus proliferation, the best combination is 6-BA and NAA, and the best recipe of callus proliferation culture medium is MS+6-BA(2. 0 mg/L) +NAA(0. 05 mg/L).关键词
攸县油茶/花药培养/愈伤组织诱导率/增殖效果Key words
Camellia yuhsiensis/ anther culture/ frequency of callus induction/ callus multiplication分类
农业科技引用本文复制引用
范晓明,袁德义,谭晓风,邹锋,姜志娜,王晓红,曾艳玲..油茶花药离体培养影响因子研究[J].南京林业大学学报（自然科学版）,2011,35(5):125-128,4.基金项目
"十一五"国家科技支撑计划(2009BADB1B0403) （2009BADB1B0403）
湖南省研究生科研创新项目(CX2010B316) （CX2010B316）
中南林业科技大学研究生科技创新基金资助项目(2008sx02) （2008sx02）
