中国农业科学2011，Vol.44Issue(15)：3206-3213,8.
荧光辅助糖电泳检测葡甘露寡糖条件的分析
Analysis of Glucomanno-Oligosaccharides by Fluorophore-Assisted Carbohydrate Electrophoresis
摘要
Abstract
[Objective] The objective of this study was to explore the conditions of FACE detecting glucomannooligosaccharides. [Method] Konjac glucomanno-oligosaccharides was used as the substrate, the optimal parameters were determined by investigating the effects of 3 kinds of fluorescer, the concentration of resolving gel, the conditions of derivatization reaction and sample impurities on FACE. [Result] The optimal conditions of FACE used in detecting glucomanno-oligosaccharides were as follows: fluorescer ANTS, concentration of resolving gel 25%, content of ANTS 2 uL, derivation reaction temperature 37°C and derivation reaction time 16 h. [Conclusion] These findings show that FACE has a high resolution to glucomannooligosaccharides, and 14 kinds of components were detected by FACE, which provides an effective method for qualitative and quantitative analysis of glucomanno-oligosaccharides.关键词
葡甘露寡糖/荧光辅助糖电泳/ANTS/检测Key words
glucomanno-oligosaccharides/ FACE/ ANTS/ detecting引用本文复制引用
姚喜梅,石波,刘程程,梁平,李静梅..荧光辅助糖电泳检测葡甘露寡糖条件的分析[J].中国农业科学,2011,44(15):3206-3213,8.基金项目
国家"863" 计划项目(2006AA10Z337和2007AA091601-2) （2006AA10Z337和2007AA091601-2）
