分析测试学报2011，Vol.30Issue(10)：1179-1182,4.DOI:10.3969/j.issn.1004-4957.2011.10.020
气相色谱-串联质谱法测定不同产地大米中的角鲨烯
Determination of Squalene in Rices from Different Areas by Gas Chromatography-Tandem Mass Spectrometry
李红 1田福林 1刘成雁 1程光荣1
作者信息
- 1. 辽宁省分析科学研究院,辽宁省标准化体系建设工程技术研究中心,辽宁,沈阳,110015
- 折叠
摘要
Abstract
Different extraction methods of bioactive component squalene in rices were studied and a new method was developed for the determination of squalene in rices. The squalene in rices from different areas were extracted by Soxhlet extract method, and analyzed by gas chromatography - tandem mass spectrometry(GC -MS/MS). The results showed that the calibration curve was linear over the concentration of squalene in the range of 1 -50 mg/L with a correlation coefficient of 0. 998 7. The detection limit( LOD) was 0. 1 mg/kg. The recoveries of squalene at two spiked levels of 5 mg/kg and 20 mg/kg were in the range of 96% - 104% with relative standard deviations ( RSDs) no more than 3. 6% . The method was sensitive and reproducible, and could be used to distinguish effectively rices with different species, origins and qualities.关键词
气相色谱-质谱/角鲨烯/大米/索氏提取Key words
gas chromatography -tandem mass spectrometry(GC -MS/ MS)/ squalene/ rice/ Soxhlet extract method分类
化学化工引用本文复制引用
李红,田福林,刘成雁,程光荣..气相色谱-串联质谱法测定不同产地大米中的角鲨烯[J].分析测试学报,2011,30(10):1179-1182,4.
