中国农业科学2011，Vol.44Issue(15)：3235-3245,11.
济宁青山羊发情周期不同阶段LHR在输卵管的分布及其mRNA表达
Localization and Differential Expression of LHR and LHR mRNA in the Oviduct of Jining Gray Goat During Estrous Cycle
摘要
Abstract
[Objective] The objective of the experiment is to study the localization of LH receptor (LHR) and the expression of LHR Mrna in the oviduct during estrous cycle in Jining Gray Goat. [Method] The target fragment of LHR Mrna was retrieved, cloned and analyzed by using the real time fluorescence quantitative PCR, then the differences in expression were detected. At the same time, the immunohistochemical staining of LHR was done. [Result] The localizations of LHR immunoreactive substance in all parts of the oviduct at different stages of estrous cycle were carried out respectively, and it was found that LHR is mainly distributed on the epithelium of tubal mucosa, lamina propria and blood vessel endothelium. LHR Mrna expression was different in the oviduct of Jining Gray Goat at the four estrous cycle phrases. LHR Mrna in the linfundibulum was the highest during estrus (P<0.01). In the ampolla, was the lowest during metestrus (P<0.05). In the isthmus, was the highest during proestrus (P< 0.01). [ Conclusion ] The localization of LHR and its Mrna expression in the oviduct of goat reveals that LH has a kind of administration on the function of ovidct.关键词
济宁青山羊/黄体生成素受体/荧光定量RT-PCR/免疫组化/输卵管Key words
Jining Gray Goat/ LHR/ RT-PCR/ immunohistochemistry/ oviduct引用本文复制引用
李广君,王慧,王树迎,侯衍猛,黄丽波..济宁青山羊发情周期不同阶段LHR在输卵管的分布及其mRNA表达[J].中国农业科学,2011,44(15):3235-3245,11.基金项目
国家自然科学基金项目(30871832)、山东省科技攻关项目(2006GG2209004) （30871832）
