中国农业科学2011，Vol.44Issue(15)：3264-3271,8.
GnRH、OT、SYN及S-100蛋白在卡拉库尔羊周期黄体细胞中的共存
Study on the Coexistence of GnRH,OT,SYN and S-100 Protein in the Same Luteal Cell of the Mid Phases of Oestrous Cycle in Karakul Sheep
摘要
Abstract
[Objective] The experiment was conducted to study coexistence of gonadotropin-releasing hormone (GnRH), oxytocin (OT), synaptophysin (SYN) and S-100 protein of dispersed or diffuse neuroendocrine system (dispersed or diffuse neuroendocrine system, DNES) in the same luteal cell. [Method] The corpus luteum was stained using pairwise combinations of OT, GnRH, SYN, and S-100 monoclonal antibody through double labeling immunofluorescence staining, respectively, at the mid phases of oestrous cycle of the Karakul sheep. [Result] Five kinds of immunofluorescence staining double positive cells combinations (OT and GnRH, OT and S-100, OT and SYN, GnRH and S-100, and GnRH and SYN) were respectively presented in luteal cell. In addition, the correlation analysis on 10 combinations of 5 kinds of double positive cells showed that there were 9 highly correlated combinations (r>0.7). [Conclusion] This result reveals that there are DNES cells widespread in the same luteal cells, and there are OT and GnRH widespread in DNES cells. So, DNES cells may be the secretion cells of OT, and GnRH, and there are dual properties of both DNES cells and endocrine cells in luteal cells.关键词
GnRH/OT/SYN/S-100/黄体细胞/共存Key words
GnRH/OT/SYN/S-100/luteal cell/coexistence引用本文复制引用
郑毛亮,李广..GnRH、OT、SYN及S-100蛋白在卡拉库尔羊周期黄体细胞中的共存[J].中国农业科学,2011,44(15):3264-3271,8.基金项目
陕西省科技创新工程项目(2007ZDKG-06) （2007ZDKG-06）
