水产学报2011，Vol.35Issue(6)：838-845,8.DOI:10.3724/SP.J.1231.2011.17247
雌二醇、壬基酚、多氯联苯、镉和锌及其混合物对唐鱼的雌激素效应比较
Comparative study of estrogenic effects of estradiol,nonylphenol,polychlorinated biphenyls,cadmium,zinc and its mixtures on Tanichthys albonubes
摘要
Abstract
Environmental endocrine disrupting chemicals (EDCs) can affect the normal endocrine system of organism. Its huge threat to the environment and the health of human beings arouses international attention, which has been the hot topic in environmental research. Vitellogenin( Vtg) production in male and juvenile fish can provide a sensitive biomarker of reproductive disruption to indicate and estimate estrogenicity of EDCs. It has been proved that the vitellogenin of Tanichthys albonubes is a perfect biomarker to be used to monitor EDCs in environment. The objective of the present study was to detect the estrogenic responses of estradiol-17 beta(E2) ,nonylphenol(NP),polychlorinated biphenyls(PCBs) ,Cd2+ ,Zn2+and its mixture in T. albonubes. Native-polyacrylamide gel electrophoresis ( Native-PAGE), lipo-staining method, indirect enzyme-linked immunosorbent assay(ELISA) were used to analyze the whole body homogenate from treated male T. albonubes. The results of Native-PAGE showed that after exposure to 10μg/L E2,50 μg/L E2 for 7 days or 14 days, the male T. albonubes could synthesize Vtg. It also showed that the more time, the more vitellogenin and the higher the concentration of E2, the more Vtg. The results of ELISA showed that the male T. albonubes could be induced to synthesize Vtg after exposure to 0.1,0. 5,1.0 |xg/L E2for 7,14 days or exposure to 200,300 μg/L NP for 14 days. The combined effects of NP,PCB,Cd2+ or Zn2 + together with different level E2 were dissimilar with their individual effects. The interaction of NP and E2was synergism action. This article also discussed the combined effects of environmental estrogen mechanism.关键词
唐鱼/内分泌干扰物/卵黄蛋白原(Vtg)/Native-PAGE电泳Key words
Tanichthys albonubes/ environmental endocrine disrupting chemicals ( EDCs) / vitellogenin (Vtg) / Native-polyacrylamide gel electrophoresis (Native-PAGE)分类
生物科学引用本文复制引用
杨丽丽,张晶,方展强..雌二醇、壬基酚、多氯联苯、镉和锌及其混合物对唐鱼的雌激素效应比较[J].水产学报,2011,35(6):838-845,8.基金项目
广东省科技计划项目(200913030600006):国家科技支撑项目(2009BAD132130401-02) （200913030600006）
