郑州大学学报（医学版）2011，Vol.46Issue(5)：681-684,4.
复方大青叶注射液对体外培养HT-H9细胞CXCR4启动子活性的影响
Effect of compound Folium Isatidis injection on activity of CXCR4 promoters in HT-H9 cells in vitro
摘要
Abstract
Aim :To investigate the effect of the compound Folium Isatidis injection on the activity of CXCR4 promoters in HT-H9 cells cultivated in vitro. Methods:The sequence of CXCR4 promoter gene was cloned,and inserted into the reporter vector pGL4. 17 to construct luciferase reporter vector (pGL4. 17-CXCR4) ,the recombinant plasmids were transfect-ed into HT-H9 cells. Screened by G418, the lasting transfected cells were allocated into 4 groups: high dose group and low dose group (treated with 200,100 mg/L compound Folium Isaticlis injection for 24 h, respectively ) , injection water control group,blank control group,and untransfection group. The luciferase activity was evaluated. Results:The results of restrict double digestions and DNA sequencing proved that pGL4. 17-CXCR4 was constructed successfully. The level of luciferase activity among the 5 groups had significant differences (F = 135.510,P＜0.001). The levels of the compound Folium Isati- . Dis injection groups, both high dose group and low dose group, were remarkably lower than those of control groups (P＜ 0.01). Conclusion: The compound Folium isatidis injection could obviously restrain the activity of CXCR4 promoters in HT-H9 cells in vitro.关键词
复方大青叶注射液/CXCR4启动子/艾滋病/HT-H9细胞Key words
compound Folium Isatidis injections/CXCR4 promoter/AIDS/HT-H9 cells分类
医药卫生引用本文复制引用
冷弘,刘慧涛,臧玉翠,马云云,李敏,靳静,赵国强..复方大青叶注射液对体外培养HT-H9细胞CXCR4启动子活性的影响[J].郑州大学学报（医学版）,2011,46(5):681-684,4.基金项目
河南省医学科技攻关基金资助项目 2008002002 （）
