中国医科大学学报2011，Vol.40Issue(7)：580-583,4.DOI:CNKI:21-1227/R.20110705.1000.001
原癌基因USP22启动子的克隆及转录活性分析
Cloning of the USP22 Oncogene Promoter and Analysis of Its Transcription Activity
摘要
Abstract
Abstract Objective To clone a part of the promoter region of human USP22 oncogene.and to investigate its transcription activity. Methods We identified the USP22 transcription start site (TSS) by 5' RACE (5' rapid amplification of cDNA ends) analysis,and then amplified its 5' UTR fragment (-2 828-+52) containing TSS. The PCR products were inserted into luciferase reporter vector pGL3-Basic. The recombinant plasmids were co-transfected into HepG2 cells and Hela cells with plasmid pRL-TK. The activities of luciferase were measured by dual luciferase reporter system. Results The USP22 gene's TSS was determinated. The electrophoresis analysis of restriction enzyme digestion and DNA sequencing verified that the fragment (-2 828-+52) was successfully amplified and inserted into pGL3-Basic. After the recombinant plasmids transfected, luciferase was highly expressed in HepG2 cells and Hela cells. Conclusion The recombinant vector containing LJSP22 promoter is constructed successfully,which provides a basis for further exploring the regulation mechanism of USP22 expression.关键词
USP22基因/启动子/报告基因Key words
Key words USP22 gene/ promoter/ report gene分类
医药卫生引用本文复制引用
熊建军,周英妹,干丽君,龚帧,林玲..原癌基因USP22启动子的克隆及转录活性分析[J].中国医科大学学报,2011,40(7):580-583,4.基金项目
国家自然科学基金资助项目(31000581) （31000581）
江西省科技支撑计划资助项目(2010BSA14000) （2010BSA14000）
江西省自然科学基金资助项目(2010GQY0199) （2010GQY0199）
