郑州大学学报（医学版）2011，Vol.46Issue(5)：684-686,3.
重组人生长激素对人巨噬细胞分泌IL-1、IL-6和TNF-α的影响
Effect of recombinant human growth hormone on production of IL-1, IL-6, and TNF-α from human macrophages
摘要
Abstract
Aim: To investigate the effects of recombinant human growth hormone ( rhCH) on the production of IL-1, IL-6, and TNF-α from human macrophages. Methods: The mononuclear cells were obtained from human peripheral blood and induced to macrophages by granulocyte and monocyte colony stimulating factor,and identified by flow cytometry. Then the macrophages were allocated into 6 groups and stimulated by lipopolysaccharide and 0,1,4,20, and 50 μg/L rhGH, respectively. The production of IL-1, IL-6, and TNF-α from macrophages were detected by ELISA. Results;The macrophages were obtained and identified. It was showed that rhGH could promote macrophages to secrete IL-1, IL-6, and TNF-α, and the contents of IL-1,IL-6,and TNF-α increased with culture time. Conclusion:rhGH could promote the production of IL-1,IL-6 and TNF-α by macrophages, suggesting that it may be involved in immune regulation.关键词
生长激素/IL-1/IL-6/TNF-α/巨噬细胞Key words
growth hormone/ IL-1 /IL-6/TNF-α/ macrophage分类
医药卫生引用本文复制引用
丁培杰,段长恩,张世杰,赵国强..重组人生长激素对人巨噬细胞分泌IL-1、IL-6和TNF-α的影响[J].郑州大学学报（医学版）,2011,46(5):684-686,3.基金项目
河南省教育厅自然科学研究计划基金资助项日 教科外[ 2008] 78号2008 C310003 （）
