首页|期刊导航|中华医学杂志（英文版）|Human dental pulp stem cell is a promising autologous seed cell for bone tissue engineering
中华医学杂志（英文版）2011，Vol.124Issue(23)：4022-4028,7.DOI:10.3760/cma.j.issn.0366-6999.2011.23.033
Human dental pulp stem cell is a promising autologous seed cell for bone tissue engineering
Human dental pulp stem cell is a promising autologous seed cell for bone tissue engineering
摘要
Abstract
Background The seed cell is a core problem in bone tissue engineering research.Recent research indicates that human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts in vitro,which suggests that they may become a new kind of seed cells for bone tissue engineering.The aim of this study was to evaluate the osteogenic differentiation of hDPSCs in vitro and bone-like tissue formation when transplanted with three-dimensional gelatin scaffolds in vivo,and hDPSCs may become appropriate seed cells for bone tissue engineering.Methods We have utilized enzymatic digestion to obtain hDPSCs from dental pulp tissue extracted during orthodontic treatment.After culturing and expansion to three passages,the cells were seeded in 6-well plates or on three-dimensional gelatin scaffolds and cultured in osteogenic medium.After 14 days in culture,the three-dimensional gelatin scaffolds were implanted subcutaneously in nude mice for 4 weeks.In 6-well plate culture,osteogenesis was assessed by alkaline phosphatase staining,Von Kossa staining,and reverse transcription-polymerase chain reaction (RT-PCR) analysis of the osteogenesis-specific genes type I collagen (COL l),bone sialoprotein (BSP),osteocalcin (OCN),RUNX2,and osterix (OSX).In three-dimensional gelatin scaffold culture,X-rays,hematoxylin/eosin staining,and immunohistochemical staining were used to examine bone formation.Results In vitro studies revealed that hDPSCs do possess osteogenic differentiation potential.In vivo studies revealed that hDPSCs seeded on gelatin scaffolds can form bone structures in heterotopic sites of nude mice.Conclusions These findings suggested that hDPSCs may be valuable as seed cells for bone tissue engineering.As a special stem cell source,hDPSCs may blaze a new path for bone tissue engineering.关键词
dental pulp stem cells/bone tissue engineering/osteogenic differentiation/gelatin scaffoldsKey words
dental pulp stem cells/bone tissue engineering/osteogenic differentiation/gelatin scaffolds引用本文复制引用
LI Jing-hui,LIU Da-yong,ZHANG Fang-ming,WANG Fan,ZHANG Wen-kui,ZHANG Zhen-ting..Human dental pulp stem cell is a promising autologous seed cell for bone tissue engineering[J].中华医学杂志（英文版）,2011,124(23):4022-4028,7.基金项目
This study was supported by a grant from the Scientific Research Fund of Beijing Friendship Hospital Affiliated to Capital Medical University (No.2009026). （No.2009026）
