中国卒中杂志2011，Vol.6Issue(11)：864-868,5.
皮层电刺激联合康复治疗对大鼠脑缺血皮层运动区突触超微结构及结构参数的影响
Ultrastructure Changes and Texture Parameters Analyses of the Synapses in Rats of Cortical Electrical Stimulation Combined with Rehabilitative Training Following Focal Cortical Ischemia
摘要
Abstract
Objective To explore the infrastructure and texture parameters changes of the synapses due to cortical electrical stimulation combined with rehabilitative training following focal cortical ischemia in rats.Methods Thirty rats reached for single food pellets in a box made of clear plexiglas. When the success rate was more 30% in three consecutive days, rats were admitted. There were eighteen rats into the group and the skilled forelimb was recorded after seven days training.Locating the brain motor area by the rat brain stereotactic atlas, and then Endothelin was injected into the starting point of the middle cerebral artery to block the blood supply of the middle cerebral artery to make infarction rat model. And then put the stimulating electrode outside the scleromeninx corresponding infarct. After operation, rats were randomly divided into two groups:stimulating group received cortical electrical stimulation combined with rehabilitative training; control group, only the rehabilitative training, which last for two weeks. The ultrastucrure changes of the synapses were observed with electron microscopy. The number of synapses and Width of synaptic cleft were studied with image analysis.Results ①There were three death after operation due to postoperative infection; ②There was nostatistical significance between the two groups before operation(P=0.144). The stimulation grouphad significantly greater rates of improvement with the impaired forelimb in comparison to controlgroup(P=0.021).③Image J software using electron microscopy image analysis results showed thatstimulation of cerebral ischemia in rat motor cortex than in the control group increased the numberof synapses, a statistically significant difference(/'<0.05). Width of synaptic cleft was no significantdifference between the two groups(f>0.05).Conclusion Cortical electrical stimulation combined with rehabilitative training following focalcortical ischemia can promote synaptic growth and enhancs plasticity of synaptic structure, thuscontributing to the rehabilitation of the hemiplegic limb function.关键词
脑梗死/康复/经皮神经电刺激/突触/神经元可塑性Key words
Brain infarction/ Rehabilitation/ Transcutaneous electric nerve stimulation/Synapses/ Neuronal plasticity引用本文复制引用
谢瑞禄,孙异临,郑建,杨力军,王硕,赵继宗,曹勇..皮层电刺激联合康复治疗对大鼠脑缺血皮层运动区突触超微结构及结构参数的影响[J].中国卒中杂志,2011,6(11):864-868,5.基金项目
国家自然科学基金面上项目:皮层电刺激联合康复治疗对大鼠脑缺血模型运动区突触可塑性的作用及机制(30973112) （30973112）
