中国当代儿科杂志2011，Vol.13Issue(11)：878-882,5.
先天性肾积水患儿肾脏水通道蛋白表达与肾实质厚度和肾小球滤过率的相关性
Correlation of renal AQP1-4 protein expression with renal parenchyma thickness and glomerular filtration rate in children with congenital hydronephrosis
摘要
Abstract
Objective To study the relationship of renal aquaporin -1, -2, -3, and -4 (AQP1-4) expression with renal parenchymal thickness and glomerular filtration rate ( GFR) in children with congenital hydronephrotis. Methods Renal tissue samples were obtained from 10 kidneys of 10 children (age; 62. 3 ± 18. 3 months) with hydronephrosis and who underwent Anderson-Hynes pyeloplasty. Renal control samples were obtained from 6 children ( age; 62. 7 ± 17. 1 months) undergoing nephrectomy for nephroblastoma and were confirmed histologically as normal renal tissues. Renal parenchymal thickness of the hydronephrotic kidneys was measured by ultrasound preoperatively and was verified at operation. Renal GFR was assessed using 99mTc-DTPA scintigraphy preoperatively. Western blot was used to examine the expression of AQP1-4 in the renal tissues. The correlations of renal AQP1-4 expression with the renal parenchymal thickness and GFR were assessed by Pearson correlation analysis. Results The expression of AQP1 -4 in the hydronephrotis group was markedly reduced compared to that in the control group ( P < 0. 05 ) . The mean renal parenchymal thickness of the hydronephrotic kidney was 4.59 ±2. 25 mm measured by ultrasound preoperatively. The mean GFR of the obstructed kidney was significantly lower than that of the contralateral kidney in the hydronephrosis group (40 ±12 mL/min vs 105 ±20 mL/min; P<0.05). The expression of AQP1,2,3 and 4 was positively correlated with preoperative renal GFR and renal parenchymal thickness in the hydronephrosis group (P < 0. 05). Renal parenchymal thickness was positively correlated with renal GFR (P<0.05). Conclusions The expression of renal AQP1-4 is reduced in children with congenital hydronephrosis. The expression levels of AQP1A are positively correlated with renal parenchymal thickness and GFR.关键词
水通道蛋白/先天性肾积水/肾实质厚度/肾小球滤过率/儿童Key words
Aquaporin/ Congenital hydronephrosis/ Renal parenchymal thickness/ Glomerular filtration rate/ Child分类
医药卫生引用本文复制引用
李真珍,王志敏,邢璐,张红,文建国..先天性肾积水患儿肾脏水通道蛋白表达与肾实质厚度和肾小球滤过率的相关性[J].中国当代儿科杂志,2011,13(11):878-882,5.基金项目
国家自然科学基金(批准号:30571931) （批准号:30571931）
河南省医学科技攻关计划资助项目(200801002) （200801002）
郑州市技术研究与开发经费支持项目(074SGHH32262). （074SGHH32262）
