新医学2011，Vol.42Issue(10)：655-660,6.DOI:10.3969/g.issn.0253-9802.2011.10.010
姜黄素提高白血病KG1a细胞对同种异体NK细胞杀伤的敏感性及其分子机制探讨
Effects of ALLO-NK cell cytotoxicity against leukemic cell lines KG1a treated with Curcumine and their molecular mechanism
摘要
Abstract
Objective; To study the effects of ALLO-NK cell cytotoxicity against leukemic cell lines Kgla treated with Curcumine and to preliminarily explore their molecular mechanisms. Methods: Taking ALLO-NK highly sensitive K562 cells as controls, cytotoxicity of NK cells isolated from 5 healthy volunteers against two leukemia cells was analyzed by LDH releasing assay at different effector-to-target cell ratios (E: T). The expressions of NKG2D ligands and HLA-class I molecule were assayed by flow cytometery; the mRNA expression of NKG2D lig-ands in two kind of cells was measured by RT-PCR; CCK-8 test was conducted to find out the 50 % inhibition concentration of Curcumine and this level of Curcumine was chosen to treat KG1 a cells; cytotoxicity of ALLO-NK cells was analyzed by LDH releasing assay and the expressions of NKG2D ligands and HLA-class I molecule were assayed by flow cytometery after treating with Curcumine once again. Results: Kgla cells is not sensitive to ALLO-NK cells. The expressions of NKG2D ligands were positively detected in the two cells at mRNA level, but they were significantly higher in K562 cells than Kgla cells at the protein level(P <0. 05). However, the expression of NKG2D ligands in Kgla cells was up-regulated(P< 0.05) and no significant difference of expression of HLA-classI molecules was observed after treateing with Curcumine(P >0.05). Conclusion; Curcumine could obviously up-regu-late NKG2D ligands expression in Kgla cells and thus enhance the cytotoxicity of NK cells against Kgla cells.关键词
姜黄素/白血病KG1a细胞/白血病K562细胞/自然杀伤细胞/NKG2D配体/细胞毒性实验Key words
Curcumine/ Leukemic cell lines Kgla/ Leukemic cell lines K562/ Natural killer cell/ NKG2D ligands/ Cytotoxicity引用本文复制引用
陈伟,刘玉莹,杜苑苑,王玲..姜黄素提高白血病KG1a细胞对同种异体NK细胞杀伤的敏感性及其分子机制探讨[J].新医学,2011,42(10):655-660,6.基金项目
2010年建设中医药强省立项资助课题(2010242) （2010242）
