南方医科大学学报2011，Vol.31Issue(11)：1890-1894,5.DOI:44-1627/R.20111025.1646.013
低氧对人白血病细胞株HL-60细胞增殖及HIF-1α表达的影响
Effect of hypoxia on the proliferation and hypoxia inducible factor-1α expression in human leukemia HL-60 cells
摘要
Abstract
Objective To establish a method based on molecular beacon real-time PCR for detecting single nucleotide polymorphisms (SNP) in codon 72 of scar-related p53 gene. Methods Two fluorescence-labeled molecular beacon probes were synthesized targeting CCC/CGC SNP of p53 codon 72. The genomic DNA was extracted from the peripheral blood of 28 patients with keloid, and the CCC/CGC SNP of P53 gene codon 72 were assayed with molecular beacon real-time PCR. The results of SNP typing were compared with the results of reverse dot hybridization and confirmed by direct DNA sequencing. Results The goodness of fit of this method was 100% in comparison with direct DNA sequencing, higher than that of reverse dot hybridization. Conclusion Molecular beacon real-time PCR is suitable for rapid clinical detection of SNPs in p53 gene.关键词
低氧诱导因子/HL-60细胞/白血病/氯化钴Key words
p53 gene/ single nucleotide polymorphism/ molecular beacon/ real-time PCR/ scar分类
医药卫生引用本文复制引用
张亚莉,徐琳,邱健,李志樑,王剑青,李锐,刘会,朱红敏..低氧对人白血病细胞株HL-60细胞增殖及HIF-1α表达的影响[J].南方医科大学学报,2011,31(11):1890-1894,5.基金项目
贵州省科技计划项目(黔科合[2010]3152号) （黔科合[2010]3152号）
中国博士后科学基金特别资助(2010003745)和一等资助(20080440215) （2010003745）
广东省自然科学基金(7000068) （7000068）
广东省和广州市科技计划项目(2009B011000018 （）
2005B3060101016和2009J1-c491) （）
