基础医学与临床2011，Vol.31Issue(11)：1256-1260,5.
NOGGIN基因过表达对MDA-MB-231乳腺癌细胞系增殖和侵袭的影响及机制
The role of NOGGIN gene overexpression in proliferation and invasion of breast cancer cell line MDA-MB-231
摘要
Abstract
Objective To investigate the effection and mechanism of NOGGIN protein in proliferation and invasion of human breast cancer cell MDA-MB-231. Methods MDA-MB-231 cells were infected with recombinant NOGGIN adenovirus. MTT, Wound-healing experiment and Matrigel invasion assays were performed to examine the change of proliferation, movement and invasion. The expression of MMP1 and CXCR4 was evaluated by real-time PCR and Western blot. ResultsThe growth of MDA-MB-231 cells infected with Ad-NOGGIN was faster than that of the control (P < 0. 05), while scratch repair time extended. Transwell cell invasion assay showed that the invasive ability of MDA-MB-231 cell was inhibited in NOGGIN group (79 ±4), compared with GFP group ( 135 ±7) (P<0. 05). Using real-time PCR and western blot, the expression of CXCR4 and MMP1 was confirmed to decrease. Conclusion NOGGIN protein can enhance the proliferation of MDA-MB-231 cell, and inhibit its invasion and metastases. Maybe the cancer-related gene CXCR4 and MMP1 are involved.关键词
NOGGIN/乳腺癌/骨转移肿瘤/MDA-MB-231Key words
NOGGIN/breast cancer/osseous metastasis/MDA-MB-231分类
医药卫生引用本文复制引用
黄佳祎,吴迪,李红丽..NOGGIN基因过表达对MDA-MB-231乳腺癌细胞系增殖和侵袭的影响及机制[J].基础医学与临床,2011,31(11):1256-1260,5.基金项目
重庆市卫生局科研课题(2010-2-157) （2010-2-157）
