基础医学与临床2011，Vol.31Issue(11)：1273-1277,5.
催产素体外诱导大鼠骨髓间充质干细胞向心肌细胞分化
Oxytocin induces rat bone marrow mesenchymal stem cells differentiate into cardiomyocyte-like cells in vitro
王海萍 1张雷 2王浩宇 1吕洋 1陈炜 2邵素霞2
作者信息
- 1. 河北北方学院组织学与胚胎学教研室,张家口075000
- 2. 河北医科大学组织学与胚胎学教研室,石家庄050017
- 折叠
摘要
Abstract
Objective To investigate the feasibility of inducing rat bone marrow mesenchymal stem cells ( MSCs) differentiating into cardiomyocytes by oxytocin(OT). Methods SD rat MSCs were isolated from rat bone marrow and cultured, then induced by OT for 72 h. The laser scanning confocal microscope (LSCM) was used to detect the expression of desmin, a-sarcomeric actin and C-TnT. GATA-4 and a-MHC expression were detected by RT-PCR. Results The cultured cells grew stablely and proliferated faster, mainly appeared to be fusiform and fibro-blastic morphology. After induced by OT for one week, most cells became spindle-like and pillar-form with oval nuclei in the center of cells and similar to cardiomyocytes. The direction of the cell arraying was identical gradually. After four weeks, a majority of cells enlarged, lengthened, showed stick-like shape and paralleled closely. MSCs induced by OT could be identified by the positive staining for desmin, a-sarcomeric actin and C-TnT. Laser scanning confocal microscopy indicated that desmin (a-sarcomeric actin) appeared in red, cTnT was green. When they were both been showed,it changed to yellow. RT-PCR assessment showed that the differentiated cells began to express GATA-4 from day 7 to day 28 of differentiation and began to express a-MHC from day 21 to day 28 of differentiation. Conclusions MSCs can differentiate into cardiomyocytes in vitro after being induced by OT.关键词
骨髓间充质干细胞/催产素/心肌细胞/细胞分化/大鼠Key words
marrow mesenchymal stem cells/ Oxytocin/ cardiomyocytes/ differentiation/ rat分类
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王海萍,张雷,王浩宇,吕洋,陈炜,邵素霞..催产素体外诱导大鼠骨髓间充质干细胞向心肌细胞分化[J].基础医学与临床,2011,31(11):1273-1277,5.
