催产素体外诱导大鼠骨髓间充质干细胞向心肌细胞分化OA北大核心CSCDCSTPCD
Oxytocin induces rat bone marrow mesenchymal stem cells differentiate into cardiomyocyte-like cells in vitro
目的 探索催产素(OT)体外诱导骨髓间充质干细胞(MSCs)向心肌细胞分化的可行性.方法 取SD大鼠四肢骨骨髓,分离培养MSCs,应用OT定向诱导,相差显微镜观察细胞形态学变化,应用免疫细胞化学、激光扫描共聚焦显微镜技术检测结蛋白,α-横纹肌肌动蛋白、心肌特异性肌钙蛋白T的表达；以半定量RT-PCR方法在诱导后7、21和28 d检测细胞心肌早期转录因子GATA-4和心肌特异性肌凝蛋白重链α-MHC的表达.结果 体外分离培养的细胞生长稳定,增殖较快…查看全部>>
Objective To investigate the feasibility of inducing rat bone marrow mesenchymal stem cells ( MSCs) differentiating into cardiomyocytes by oxytocin(OT). Methods SD rat MSCs were isolated from rat bone marrow and cultured, then induced by OT for 72 h. The laser scanning confocal microscope (LSCM) was used to detect the expression of desmin, a-sarcomeric actin and C-TnT. GATA-4 and a-MHC expression were detected by RT-PCR. Results The cultured cells grew stabl…查看全部>>
王海萍;张雷;王浩宇;吕洋;陈炜;邵素霞
河北北方学院组织学与胚胎学教研室,张家口075000河北医科大学组织学与胚胎学教研室,石家庄050017河北北方学院组织学与胚胎学教研室,张家口075000河北北方学院组织学与胚胎学教研室,张家口075000河北医科大学组织学与胚胎学教研室,石家庄050017河北医科大学组织学与胚胎学教研室,石家庄050017
医药卫生
骨髓间充质干细胞催产素心肌细胞细胞分化大鼠
marrow mesenchymal stem cells Oxytocin cardiomyocytes differentiation rat
《基础医学与临床》 2011 (11)
1273-1277,5
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