解放军医学院学报2011，Vol.32Issue(11)：1139-1141,3.DOI:11-3275/R.20110627.0929.002
携带SV40T/HSV-tk基因永生化正常成人肝细胞的构建
Construction of immortalized human liver cells with SV40T/HSV-tk gene
摘要
Abstract
Objective To provide safe and effective artificial liver human liver cells by constructing immortalized normal adult liver cells. Methods Artificial liver human liver cells were transfected and packaged to obtain retrovirus which was used to infect the normal adult liver cells HL-7702. Liver cells with the HSV-tk gene were detected by ganciclovir toxicity test. SV40T mRNA-transfected liver cells were detected by RT-PCR. SV40T gene expression in transfected liver cells was detected by Western blot with immunofiuorescence staining. Results Ganciclovir toxicity test showed that the transfected liver cells survived well while the untransfected liver cells died. Immortalized SV40T mRNA was detected by RT-PCR in the transfected liver cells, while SV40T protein was detected by Western blot in the transfected liver cells with immunofiuorescence staining. Conclusion SV40T and HSV-tk genes can be detected in transfected normal adult liver cells and express their associated proteins.关键词
永生化/正常成人肝细胞/逆转录病毒/自杀基因Key words
immortalized/ normal adult liver cells/retroviral/suicide gene分类
医药卫生引用本文复制引用
姚文芳,崔忻,田磊,高宇红,洪权,薛毅珑..携带SV40T/HSV-tk基因永生化正常成人肝细胞的构建[J].解放军医学院学报,2011,32(11):1139-1141,3.基金项目
国家“863”计划项目(2006A A02Z498) （2006A A02Z498）
