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胃癌细胞中miRNA-204过表达的作用

张文辉 桂俊豪 王昌正 常青 吴本俨

解放军医学院学报2011,Vol.32Issue(11):1142-1143,1170,3.
解放军医学院学报2011,Vol.32Issue(11):1142-1143,1170,3.

胃癌细胞中miRNA-204过表达的作用

Role of miR-204 over-expression in growth and apoptosis of gastric cancer cells

张文辉 1桂俊豪 2王昌正 2常青 2吴本俨2

作者信息

  • 1. 解放军第302医院肝硬化中心胃镜室,北京100039
  • 2. 解放军总医院,北京 100853
  • 折叠

摘要

Abstract

Objective To observe the effect of miR-204 over-expression on growth and apoptosis of gastric cancer cells. Methods Expression of miR-204 in gastric cancer cells was detected by real time PCR and transfected into gastric cancer cells. Growth and apoptosis of gastric cancer cells were detected by MTS and flow cytometry, respectively. Results The expression level of miR-204 was low in gastric cancer cells, which was similar to that in gastric tissue. No significant change was observed in the growth and apoptosis of gastric cancer cells when miR-204 was over-expressed. Conclusion Over-expression of miR-204 has no significant effect on the growth and apoptosis of gastric cancer cells.

关键词

微小RNA/过表达/细胞凋亡

Key words

microRNA/over expression/apoptosis

分类

医药卫生

引用本文复制引用

张文辉,桂俊豪,王昌正,常青,吴本俨..胃癌细胞中miRNA-204过表达的作用[J].解放军医学院学报,2011,32(11):1142-1143,1170,3.

基金项目

国家科技支撑计划项目(2009BAI86B05) (2009BAI86B05)

解放军医学院学报

OACSTPCD

2095-5227

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