中国药房2011，Vol.22Issue(41)：3894-3896,3.
反相离子对高效液相色谱法测定复方苯海拉明滴鼻液中盐酸苯海拉明和盐酸麻黄碱的含量
Content Determination of Diphenhydramine Hydrochloride and Ephedrine Hydrochloride in Compound Diphenhydramine Nasal Drops by Ion-pair RP-HPLC
刘炜 1曾蔚欣 1刘礼斌 1唐宛晨 1孙路路1
作者信息
- 1. 首都医科大学附属北京世纪坛医院,北京市100038
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for the content determination of diphenhydramine hydrochloride and ephed-rine hydrochloride in Compound diphenhydramine nasal drops. METHODS: Ion-pair RP-HPLC method was adopted. Agilent ZOR-BAX Eclipse XDB-C18 column was used with 0.02 mol-L-1 sodium laurylsulfate solution (pH adjusted to 2.85 with 85% phosphate acid solution)-methanol-triethanolamine (52:48:0.5) at the flow rate of 1.0 mL·min-1. The UV detection wavelength was set at 256 nm. The column temperature was 30 TC. RESULTS: Diphenhydramine hydrochloride and ephedrine hydrochloride could be well isolated from other substances. The calibration curve was linear in the range of 0.500 2 to 5.002 0 ug for diphenhydramine hydrochloride (r=0.999 8,n=6). The calibration curve was linear in the range of 4.005 8 to 40.058 0 μg for ephedrine hydrochloride (r= 0.999 4, n=6). The average recovery of diphenhydramine hydrochloride was 100.2% (RSD=1.2% , n=9), and that of ephedrine hydrochloride was 99.5% (RSD=1.1% , n=9). CONCLUSION: The method is specific, accurate and reliable for the content determination of diphenhydramine hydrochloride and ephedrine hydrochloride in Compound diphenhydramine nasal drops.关键词
复方苯海拉明滴鼻液/盐酸苯海拉明/盐酸麻黄碱/高效液相色谱法/反相离子对/含量测定Key words
Compound diphenhydramine nasal drops/ Diphenhydramine hydrochloride/ Ephedrine hydrochloride/ HPLC/ Reverse phase ion-pair/ Content determination分类
医药卫生引用本文复制引用
刘炜,曾蔚欣,刘礼斌,唐宛晨,孙路路..反相离子对高效液相色谱法测定复方苯海拉明滴鼻液中盐酸苯海拉明和盐酸麻黄碱的含量[J].中国药房,2011,22(41):3894-3896,3.
