中国药房2011，Vol.22Issue(43)：4048-4050,3.
半夏生物碱对人肝癌细胞Bel-7402增殖的影响
Effects of the Alkaloids of Pinellia ternata on Proliferation of Human Hepatocarcinoma Cell Strain Bel-7402
陈芳 1杨李 1王晓昆 1张宜1
作者信息
- 1. 广州军区武汉总医院医学实验科,武汉市430070
- 折叠
摘要
Abstract
OBJECTIVE: To study the effect of the alkaloids of Pinellia ternata on human hepatocarcinoma cell strain Bel-7402 proliferation. METHODS: Human hepatocarcinoma cell strain Bel-7402 co-cultured with alkaloids of P. Ternata (400, 200, 100 μg· mL-1). MTT method was used to detect the effect of alkaloids of P. Ternate on human hepatocarcinoma cell strain Bel-7402 proliferation. TUNEL technique was used to detect the effect of alkaloids of P. Ternata on human hepatocarcinoma cell strain Bel-7402 apop-tosis, and immunohistochemistry SABC staining method was used to test Bax and Bcl-2 protein expression. RESULTS: The high-dose, medium-dose and low-dose of alkaloids of P. Ternata (400, 200, 100 ng · mL-1) could inhibit the proliferation of Bel-7402 cell after 24 hours treatment, the inhibitory effect enhanced as the concentration increased, the highest inhibitory rate reached 36.98% in dose-response relationship. TUNEL showed that three different doses of alkaloids of P. Ternata could induce Bel-7402 cell apoptosis in dose-response relationship. Three different doses of alkaloids of P. Ternata could increase Bax expression but decrease Bcl-2 expression. CONCLUSION: Alkaloids of P. Ternata can inhibit human hepatocarcinoma cell strain Bel-7402 proliferation and introduce its apoptosis, and its mechanism is associated with up-regulation of Bax and down-regulation of Bcl-2.关键词
半夏生物碱/Bel-7402肿瘤细胞:细胞凋亡/B细胞淋巴瘤/白血病-2/B细胞淋巴瘤/白血病-2相关X蛋白Key words
Alkaloids of Pinellia ternata/ Human hepatocarcinoma cell strain Bel-7402/ Cell apoptosis/ Bcl-2/ Bax分类
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陈芳,杨李,王晓昆,张宜..半夏生物碱对人肝癌细胞Bel-7402增殖的影响[J].中国药房,2011,22(43):4048-4050,3.
