中国药房2011，Vol.22Issue(43)：4059-4061,3.
β-葡萄糖苷酶酶解淫羊藿苷制备宝藿苷Ⅰ的工艺研究
Preparation Technology of Baohuoside I by Enzymolysis of Icariin with β-glucosidase
摘要
Abstract
OBJECTIVE: To study the preparation technology of baohuoside I by enzymolysis of icariin with β-glucosidase. METHODS: Taking byconversion rate as index,β-glucosidase was used for enzymolysis of icariin and the effects of temperature, Ph value, the ratio of enzyme to substrate, substrate concentration and reaction time on conversion rate of baohuoside I were investigated by single factor test. RESULTS: The optimum reaction conditions were determined as follows: 50 t, citric acid-citric acid sodium acetate buffer (Ph 5.0), the ratio of enzyme to substrate 1:2,substrate concentration 1 rng·Ml-1 and reaction time 90 min. CONCLUSION: It is simple and reliable to prepare baohuoside I by the enzymolysis of icariin with /7-glucosidase; the conversion rate is high and the technology is moderate.关键词
淫羊藿苷/宝藿苷Ⅰ/β-葡萄糖苷酶/生物转化Key words
Icariin/ Baohuoside I / β-glucosidase/ Bioconversion分类
医药卫生引用本文复制引用
张振海,陈玲玲,贾晓斌,贺俊杰,金鑫..β-葡萄糖苷酶酶解淫羊藿苷制备宝藿苷Ⅰ的工艺研究[J].中国药房,2011,22(43):4059-4061,3.基金项目
国家自然科学基金资助项目(30973944) （30973944）
