现代妇产科进展2011，Vol.20Issue(10)：810-813,819,5.
盆腔器官脱垂患者外周血和盆底组织中mtDNA和mtDNA4977的含量及意义
The relative quantification and significance of mtDNA and mtDNA4977 in peripheral blood and different pelvic floor tissues of patients with pelvic organ prolapse
摘要
Abstract
Objective: To investigate the relative quantification of mitochondrial DNA (mtDNA)and 4977 deletion of mtDNA ( mtDNA4977) in peripheral blood and different pelvic floor tissues of the patients with pelvic organ prolapse ( POP). Methods: Real-time PCR was used for the relative quantification of mtDNA and mtDNA4977 in peripheral blood, uterosacral ligament, vaginal anterior and posterior wall tissue of 26 POP subjects and 21 non-POP control women. Results; ( 1) In uterosacral ligament, mtDNA quantification in POP group were significantly lower than that in non-POP group(P<0.05), while in peripheral blood and vaginal anterior and posterior wall tissue, there were insignificant difference between POP and non-POP group(P>0. 05). In peripheral blood, uterosacral ligament, vaginal anterior and posterior wall tissue, mtDNA4977 quantification had a significant increase between POP and non-POP group (P <0.05 ). Whether the quantification of mtDNA or mtDNA4977, there were no significant difference between vaginal anterior and posterior wall tissue ( P>0. 05). ( 2 ) Accompanied with the advanced stage of POP (analysis between POP-0 and POP-IE stage) ,The trend towards a decrease of mtDNA quantification in uterosacral ligament was found, while in peripheral blood, vaginal anterior and posterior wall tissue, the trend was insignificant. In peripheral blood, uterosac-ral ligament, vaginal anterior and posterior wall tissue, a significantly increased proportion of mtDNA .accompanied by advanced-stage POP,was tound(P<0.05) . (3)There were no correlation between the quantification of mtDNA and mtDNA4977 in peripheral blood, uterosacral ligament and vaginal wall tissue(P>0. 05). MtDNA quantification in peripheral blood of POP patients had a positive correlation with that in uterosacral ligament (P<0. 05) , while there was no correlation of mtDNA quantification between peripheral blood and uterosacral ligament of POP patients(P>0. 05). Conclusions.-A depletion of mtDNA and an increase of mtDNA4977 may play an important role in the molecular pathogenesis of POP formation.关键词
盆腔器官脱垂/线粒体DNA/mtDNA4977/实时定量PCR法/骶韧带Key words
Pelvic organ prolapse/ Mitochondrial DNA/ mtDNA4977/ Real-time PCR/ Uterosacral ligament分类
医药卫生引用本文复制引用
赵金钗,黄向华,杜彦芳,李晓冬,张琳,尹桂然,刘义彬,王振海..盆腔器官脱垂患者外周血和盆底组织中mtDNA和mtDNA4977的含量及意义[J].现代妇产科进展,2011,20(10):810-813,819,5.基金项目
河北省医学适用技术跟踪项目(No:GL200811) （No:GL200811）
