首页|期刊导航|中华医学杂志（英文版）|Lipopolysaccharide-enhanced early proliferation of insulin secreting NIT-1 cell is associated with nuclear factor-kappaBmediated inhibition of caspase 3 cleavage
中华医学杂志（英文版）2011，Vol.124Issue(22)：3652-3656,5.DOI:10.3760/cma.j.issn.0366-6999.2011.22.010
Lipopolysaccharide-enhanced early proliferation of insulin secreting NIT-1 cell is associated with nuclear factor-kappaBmediated inhibition of caspase 3 cleavage
Lipopolysaccharide-enhanced early proliferation of insulin secreting NIT-1 cell is associated with nuclear factor-kappaBmediated inhibition of caspase 3 cleavage
摘要
Abstract
Background Increased levels of plasma lipopolysaccharide (LPS) have been found in obesity and diabetes patients.This study was to investigate the effect of LPS on pancreatic beta-cell viability and the involvement of caspase 3 in NIT-1 cell line.Methods Mouse insulinoma NIT-1 cells were treated with LPS for the indicated time and dose.Cell viability was measured by cell counting kit-8 reagent.Toll-like receptor 4 (TLR4),caspase 3 and cleaved caspase 3 were detected by Western blotting.Insulin was determined by radioimmunoassay (RIA).Results LPS promoted NIT-1 cell proliferation at 1 μg/ml,peaked at 72 hours of incubation.A reduction in cleavage of caspase 3 was observed upon LPS treatment.Bay11-7082,a specific inhibitor of nuclear factor (NF)-κB,blunted LPS-induced inhibition of caspase 3 cleavage.Reduction in chronic insulin secretion was observed after treatment with LPS at 1 μg/ml for 48 and 72 hours,not for 24 hours.TLR4 protein was upregulated when NIT-1 cells were treated with LPS at 1 pg/ml for 24 hours.Conclusions LPS promotes early NIT-1 cell proliferation in association with NF-κB-mediated inhibition of caspase 3 cleavage.LPS exerts a time-dependent inhibitory effect on chronic insulin secretion from NIT-1 cells.关键词
lipopolysaccharide/NIT-1 cell/toll-like receptor 4/nuclear factor-κB/caspase 3Key words
lipopolysaccharide/NIT-1 cell/toll-like receptor 4/nuclear factor-κB/caspase 3引用本文复制引用
LIU Shan-ying,LIANG Qi-jun,LIN Tian-xin,FAN Xin-lan,LIANG Ying,Uwe Heemann,LI Yan..Lipopolysaccharide-enhanced early proliferation of insulin secreting NIT-1 cell is associated with nuclear factor-kappaBmediated inhibition of caspase 3 cleavage[J].中华医学杂志（英文版）,2011,124(22):3652-3656,5.基金项目
This work was supported by grants from the National Natural Science Foundation of China (No.30671974 and 81070598).The authors declare no conflict of interest. （No.30671974 and 81070598）
