山东医药2011，Vol.51Issue(40)：57-60,4.
阿霉素肾病大鼠nephrin及podocalyxin蛋白表达及泼尼松干预作用的探讨
Expression of nephrin and podocalyxin in rats with adriamycin-induced nephrosis and intervention effect of prednisone
摘要
Abstract
Objective To investigate the expression of nephrin and podocalyxin in rat* with adriamycin-indueed ne-phrosis ( AND) and the intervention effect of prednisone. Methods Twenty-four male Wistar rats were randomly divided into three groups, which were AND group, prednisone group, control group. Serum cholesterol, albumin ( Alb) , and the 24-hour urine protein (24hUP) were measured serially in the 0, 4th, and 8th week. Kats were sacrificed to collect kidney tissue for pathological morphology observation in the end of study (the 8th week). Extracellular matrix/glumerular area ratio ( ECM/GA) and renal pathology points were determined. The expression of nephrin and podoealyxin in glomerulus werp examined by means of inimunohistm-hemistry. Results In the 4th week, 24hUP and cholesterol were significantly higher in AND group than those in control group respectively ( P < 0.05 ) , while Alb was significantly lower ( P < 0. 01 ). In the 8th week, compared with AND group, in prednisone group, 24hUP, cholesterol, ECM/GA, and renal pathology points were significantly lower ( P <0.01 ) , while Alb and the expression of nephrin and podocalyxin were significantly higher (P <0.0I ). The expression of nephrin and podocalyxin in AND group were negatively correlated with 24hUP, cholesterol, ECM/GA and glomerular pathology point, and positively correlated with Alb. The expression of nephrin was positively correlated with that of podocalyxin. Conclusions Nephrin and podocalyxin are closely related to the progression of proteinuri-a. The mechanism of the prednisone alleviating proteinuria and kidney pathological damage may be correlated to the up-reg-ulation of the expression of nephrin and podocalyxin.关键词
泼尼松/阿霉素肾病/nephrin/podocalyxinKey words
prednisone/adriamycin-induced nephrosis/nephrin/podocalyxin分类
医药卫生引用本文复制引用
胡小云,张碧丽..阿霉素肾病大鼠nephrin及podocalyxin蛋白表达及泼尼松干预作用的探讨[J].山东医药,2011,51(40):57-60,4.基金项目
天津市卫生局科技基金项目(03KY32). （03KY32）
