西南国防医药2011，Vol.21Issue(12)：1280-1283,4.DOI:10.3969/j.issn.1004-0188.2011.12.002
血浆循环microRNA-126在非小细胞肺癌诊断中的应用
Application of plasma circulation microRNA - 126 to diagnosis of non - small cell lung cancer
摘要
Abstract
Objective To discuss the correlation between the concentration of plasma circulation microRNA - 126 and the non - small cell lung cancer( NSCLC ), and to study its diagnostic value. Methods The concentration of plasma circulation micro RNA - 126 in 130 patients with NSCLC( NSCLC group )and 170 healthy people( control group )was detected through the method of real time fluorescent quantitation PCR. Results The concentration of microRNA - 126 in the control group was significantly higher than that in the NSCLC group( P < 0. 01 ). The area under the receiver operating characteristic curve of the concentration was 0. 758, and the 95% CI was 0. 704 - 0. 81l( P < 0. 01 ). When the concentration of plasma microRNA - 126 was 1051 fmol/L, its specificity in the diagnosis of NSCLC was 90% ,and its sensitivity was 46. 4%. There were not obvious correlations between the concentration and age, gender, smoking, pathological typing and staging of the 130 patients. Conclusion The concentration of plasma circulation microRNA -126 has certain significance in the diagnosis of NSCLC. Combined with other plasma microRNA or tumor markers, such as neuron -specific enolase,carcino - embryonic antigen and cytokeratin fragment 21 - 1, it can retrieve the limitations of the simple detection, increase the positive detection rate of lung cancer and provide dependable data for clinical diagnosis.关键词
非小细胞肺癌/循环microRNA-126/实时荧光定量PCRKey words
non - small cell lung cancer/ circulation microRNA - 126/real time fluorescent quantitation PCR分类
医药卫生引用本文复制引用
刘沙,杨季云..血浆循环microRNA-126在非小细胞肺癌诊断中的应用[J].西南国防医药,2011,21(12):1280-1283,4.基金项目
国家自然科学基金(30800633) （30800633）
四川省杰出青年基金(09ZQ026 - 034) （09ZQ026 - 034）
