生殖医学杂志2011，Vol.20Issue(5)：400-405,6.DOI:10.3969/j.issn.1004-3845.2011.05.013
兔卵巢组织慢速冷冻和玻璃化冷冻保存方法的研究
Study of slow-cooling and vitrification for rabbit ovarian tissue cryopreservation
符晓倩 1何方方 1甄璟然1
作者信息
- 1. 中国医学科学院,北京协和医学院,北京协和医院妇产科,北京100730
- 折叠
摘要
Abstract
Objective; To compare the protective effect on rabbit ovarian tissue with different cryopreservation methods.Methods; The ovarian biopsies from 15 New Zealand female rabbits were randomly divided into fresh (Group A), Dimethyl sulfoxide (DMSO) slow-cooling(Group B), propylene glycol (PROH) slow-cooling (Group C), tube vitrification (Group D) , solid-surface vitrification (Group E) groups. The rates of morphological integral follicles were compared between fresh and the post-freezing/thawing tissue. The levels of estradiol in culture medium were measured to compare the endocrine functions of the post-cryopreservation tissue. The apoptotic follicles in ovarian tissue were detected by TdT-mediated dUTP nick end labeling.Results: Compared with Group A, the percentages of morphologically normal primordial follicles significantly reduced (P<0. 05) in Group B and D, and there were no significant differences in Group C and E. The percentages of morphologically normal primary follicles were not different among 5 groups. After 14 days in-vitro culture, all cryopreservation groups showed significant reduction of the proportions of primordial follicles in cultured tissues compared with Group A (P<0. 001). The proportions of primary follicles were not different among 5 groups. The levels of estradiol in culture medium gradually increasedduring the 14 days in-vitro culture (P<0. 001). The difference of estradiol levels between slow-cooling and vitrification were significant (P = 0. 021). The percentages of apoptotic follicles were not different among all groups.Conclusion; Both slow-cooling and vitrification for rabbit ovarian tissue cryopreservation were effective, and the endocrine functions of the post-cryopreservation tissue could be preserved satisfactorily.关键词
慢速冷冻/玻璃化冷冻/卵巢组织Key words
Slow-cooling/ Vitrification/ Ovarian tissue引用本文复制引用
符晓倩,何方方,甄璟然..兔卵巢组织慢速冷冻和玻璃化冷冻保存方法的研究[J].生殖医学杂志,2011,20(5):400-405,6.
