重庆医学2011，Vol.40Issue(34)：3474-3476,3479,封3,5.DOI:10.3969/j.issn.1671-8348.2011.34.017
缺血再灌注的兔肾动脉阻力指数与肾组织TNF-α表达的相关性研究
A correlative study on the resistance index of rabbit renal artery after ischemia-reperfusion and the TNF-α expression in renal tissue
摘要
Abstract
Objective To explore the correlation of expression of tumor necrosis factor-alpha(TNF-α) in renal tissue and the resistance index (RI) of renal artery after renal ischemia-reperfusion(I/R) , and provide a feasible method to evaluate the occurrence of renal I/R injury. Methods 48 rabbits were randomly divided into sham operation group and I/R group. Color Doppler flow imaging (CDFI) was adopted to monitor the changes of RI of main renal artery (MRA) ,segmental renal artery (SRA) and interlobar renal artery (IRA) of rabbits in each group. Immunohistochemistry was employed to detect the expression of TNF-α in renal tissue. Results Compared with sham operation group, RI of renal artery showed no significant changes in rabbits subjected to 2 hours of reperfusion in I/R group,while that of SRA,IRA and that of MRA,SRA,IRA increased in rabbits subjected to 8 and 24 hours of reperfusion in I/R group,respectively(P<O. 05). Level of TNF-α expression in renal tissue of rabbits in I/R 8,24 h group was higher than that in sham operation group(P<0. 05). RI of MRA, SRA and IRA in each group had positive correlation with its TNF-a expression in renal tissue(P<0. 05). Conclusion Detection the RI of renal artery after I/R by CDFI is a feasible method with non-invasion to evaluate timely the occurrence of renal IRI.关键词
肾动脉/再灌注损伤/血管阻力/肿瘤坏死因子α/超声检查,多普勒,彩色Key words
renal artery/reperfusion injury/ vascular resistance/tumor necrosis factor-alpha/ ultrasonography,Doppler,color引用本文复制引用
陈晓梅,李明星,李昆萍,宣吉晴..缺血再灌注的兔肾动脉阻力指数与肾组织TNF-α表达的相关性研究[J].重庆医学,2011,40(34):3474-3476,3479,封3,5.基金项目
四川省卫生厅科研课题资助项目(070243). （070243）
